Neutrophils Rat Monoclonal Antibody [Clone ID: 7/4]

CAT#: CL050LE

Neutrophils rat monoclonal antibody, clone 7/4, Low Endotoxin

Product Images

Staining of Mouse peripheral blood granulocytes with Rat Anti Mouse Neutrophils- Low Endotoxin (CL050LE).

Specifications

Product Data

• Clone Name: 7/4
• Applications: FC, IHC, WB
• Recommended Dilution: Flow Cytometry: Use 10 µl of 1/50-1/100 to label 10e6 cells in 100 µl.
  Immunohistochemistry on Frozen and Paraffin Sections.
  Western blot.
  Immunoprecipitation.
• Reactivities: Mouse
• Host: Rat
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Cultured bone marrow cells.
  Spleen cells from AO rats were fused with cells from the Y3 Ag1.2.3 rat myeloma cell line.
• Specificity: This antibody recognises a polymorphic 40kD antigen expressed by polymorphonuclear cells, but absent on resident tissue macrophages.
  Strains positive for clone 7/4: 129J; AKR; C57BL/6; C57BL/10; C58; DBA/2; NZB; NZW; SJL; MFI; Swiss (PO) Strains.
  Negative/Weak for clone 7/4: A2G; A/Sn; ASW; BALB/c; C3H/HEH: CBA.T6T6.
• Formulation: PBS without preservatives.
  State: Low Endotoxin
  State: Liquid purified Ig fraction.
• Concentration: lot specific
• Purification: Affinity Chromatography on Protein G.
• Conjugation: Unconjugated
• Storage: Store the antibody undiluted at -20°C only.
  Storage in frost-free freezers is not recommended.
  Avoid repeated freezing and thawing as this may denature the antibody.
  Should this product contain a precipitate we recommend microcentrifugation before use.
• Stability: Shelf life: one year from despatch.
• Note: Endotoxin Level: < 0.01 EU/Ug