Cingulin (CGN) Guinea Pig Polyclonal Antibody

CAT#: BP5026

Cingulin (CGN) guinea pig polyclonal antibody, Serum

Product Images

Immunofluorescence staining of Cingulin in primary epithelial cells of sheep rumen using antibody BP5026 (1/100, periodate-lysin-paraformaldehyde fixation; Courtesy of S. Bilk, TiHo Hannover, German

Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution: Immunoblotting.
  Immunofluorescence Microscopy: 1/100. Dilute immedialtely with PBS before use.
  Immunohistochemistry on Frozen Sections On cultured cells initial fixation with formaldehyde is recommended for optimal staining.
  Immunohistochemistry on Paraffin Sections (Only after microwave treatment).
  Positive Control: Total cell lysate of e.g. CaCo-2 cells.
• Reactivities: Bovine, Human, Mouse, Rabbit, Xenopus
• Host: Guinea Pig
• Clonality: Polyclonal
• Immunogen: Full length GST-fusion protein of Human Cingulin
• Specificity: The antibody reacts specifically with cingulin (a ca.150 kD polypeptide) present in tight junctions (zonula occludens) of polar epithelia (e.g. colon, duodenum, kidney, liver, pancreas) of transitional epithelia (e.g. bladder epithelium) and of stratified epithelia (e.g. skin, foot sole, tongue, oesophagus, vagina). It is negative with endothelia of vessels and capillaries (e.g. in heart and brain).
  Tested Reactivities on Cultured Cell Lines: Carcinoma cell lines of Human (e.g. MCF-7, Caco-2, PLC, A-431), Bovine (MDBK, BMGE) and Canine (MDCK) origin.
• Formulation: State: Serum
  State: Liquid Stabilized Antiserum
  Preservative: 0.09% Sodium Azide
• Conjugation: Unconjugated
• Storage: Store the antibody undiluted at 2-8°C.
• Stability: Shelf life: one year from despatch.
• Gene Name: cingulin
• Database Link:
  Entrez Gene 57530 Human
  UniProt ID Q9P2M7
• Background: Cingulin, a polypeptide of 126.5 kD (calculated from aa sequence data); apparent Mr 150 kD (after SDS-PAGE); pI 6.5 (very similar to that of symplekin, another junctional protein)
• Synonyms: CGN, KIAA1319

Reference Data

• Protein Pathways: Tight junction