Neurofilament (NEFM) Mouse Monoclonal Antibody [Clone ID: NF403]

CAT#: BM5505

Neurofilament (NEFM) mouse monoclonal antibody, clone NF403, Supernatant

Specifications

Product Data

• Clone Name: NF403
• Applications: IHC, WB
• Recommended Dilution: Immunohistochemistry on Frozen and Paraffin Sections: 1/10 for 1 h at room temperature.
  Recommended Positive Control: Human brain tissue.
  Immunoblotting.
  Cell preparations.
• Reactivities: Bovine, Guinea Pig, Human, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Neurofilament preparation isolated from calf brain tissue.
• Specificity: Marker for the differentiation of 160 kD neurofilament peptide from other intermediate filament proteins.
  The antibody is useful for studying neurofilament expression and for tumor characterization (pheochromocytoma, paraganglioma, ganglioneuroblastoma, ganglioneuroma and other tumors of neuronal origin).
• Formulation: State: Supernatant
  State: Liquid Culture Supernatant containing 0.09% Sodium Azide as preservative.
• Conjugation: Unconjugated
• Storage: Store the antibody undiluted at 2-8°C.
  DO NOT FREEZE!
• Stability: Shelf life: one year from despatch.
• Gene Name: neurofilament, medium polypeptide
• Database Link:
  Entrez Gene 4741 Human
  UniProt ID P07197
• Background: Neurofilaments can be defined as the intermediate or 10nm diameter filaments found in neuronal cells. They are composed a mixture of subunits which often includes the neurofilament triplet proteins, NF-L, NF-M and NF-H. Neurofilaments may also include peripherin, alpha-internexin, nestin and in some cases vimentin. Antibodies to the various neurofilament subunits are very useful cell type markers since the proteins are quite abundant, biochemically stable. This antibody was raised against the C-terminal region of NF-M, a region highly conserved between NF-M molecules in different species. Accordingly the antibody recognizes all mammalian and avian NF-M molecules. The name NF-M refers to the fact that this is the "middle" or "medium" protein of neurofilament triplet, the other two proteins being "low" or "light" (NF-L) and "high" or "heavy" (NF-H). NF-M is sometimes also referred to as NEF3 or Neurofilament
• Synonyms: Neurofilament medium polypeptide, NF-M, NEF3, NEFM, Neurofilament 3, (Neuronal Marker)