MRP8 (S100A8) Mouse Monoclonal Antibody [Clone ID: S13.67]

CAT#: BM4029

MRP8 (S100A8) mouse monoclonal antibody, clone S13.67, Purified

Product Images

Frozen Section of Human Tonsil stained with S100A8 Antibody Clone S13.67

Specifications

Product Data

• Clone Name: S13.67
• Applications: ELISA, FC, IHC, WB
• Recommended Dilution: ELISA.
  Immunohistochemistry on Frozen Sections: 1-2 µg/ml (1/100-1/200).
  Immunohistochemistry on Paraffin Sections: 4 µg/ml (1/50).
  Pre-treatment for antigen retrieval not required.
  Has been described to work in FACS and Dot Blots.
  Suggested Positive Control: Human tonsil.
• Reactivities: Human, Porcine, Rat, Bovine
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Cultured Human monocytes.
  Antigen: MRP8.
  Epitope: Suspected in the N- or C-terminal Domain.
• Specificity: Clone S13.67 identifies MRP8: The antibody is useful in various immunological techniques. Histological and serological data indicate that MRP8 is associated with chronic stages of inflammatory diseases. This clone also stains cells in rat spleen, indiating significant cross reactivity with the corresponding Rat MRP8.
  Antigen Distribution on Isolated Cells: The antigen is found in granulocytes and monocytes. It is absent from other blood cells. In cultured monocytes, maximum MRP8 is expressed after 3-4 days.
  Antigen Distribution on Tissue Sections: In the tissue, MRP-8 is only found in a distinct subpopulation of inflammatory perivascular infiltrates of the myelo-monocytic lineage. Macrophages increasingly synthesise MRP-8 during the late stages of inflammation. A low MRP-8 (and high MRP-14) expression by macrophages was also reported in granulomatous diseases such as tuberculosis and sarcoidis. In non-granulomatous chronic inflammatory diseases such as chronic rheumatoid arthritis MRP8 and MRP14 positive cells consist of different subpopulations. During early inflammation endothelial cells are also positive with MRP8/MRP14.
• Formulation: Stock solution contains PBS, pH 7.2 with 5 mg/ml BSA as a stabilizer and 0.05% (v/v) Kathon CG as preservative.
  State: Purified
  State: Lyophilized purified IgG fraction
• Reconstitution Method: Restore by adding 0.5 ml distilled water.
• Concentration: 0.2 mg/ml (after reconstitution)
• Purification: Affinity Chromatography
• Conjugation: Unconjugated
• Storage: Store lyophilized at 2-8°C for 6 months or at -20°C long term.
  After reconstitution store the antibody undiluted at 2-8°C for one month
  or (in aliquots) at -20°C long term.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: S100 calcium binding protein A8
• Database Link:
  Entrez Gene 6279 Human
  UniProt ID P05109
• Background: MRP8 is the Ca²⁺-binding light subunit of Calprotectin. MRP8 forms Ca²⁺-dependent or complexes with MRP14 (S100A9, Calgranulin B). It also forms disulfide-linked homodimers under the influence of hypochlorite, a process thought to abrogate the chemotactic property of MRP8.
• Synonyms: S100-A8, CAGA, MRP-8, CFAG
• Note: Protocol: Protocol with frozen, ice-cold acetone-fixed sections:
  The whole procedure is performed at room temperature
  1. Wash in PBS
  2. Block endogenous peroxidase
  3. Wash in PBS
  4. Block with 10% normal goat serum in PBS for 30min. in a humid chamber
  5. Incubate with primary antibody (dilution see datasheet) for 1h in a humid chamber
  6. Wash in PBS
  7. Incubate with secondary antibody (peroxidase-conjugated goat anti mouse IgG+IgM (H+L) minimal-cross reaction to human) for 1h in a humid chamber
  8. Wash in PBS
  9. Incubate with AEC substrate (3-amino-9-ethylcarbazol) for 12min.
  10. Wash in PBS
  11. Counterstain with Mayer’s hemalum
  
  Protocol with formalin-fixed, paraffin-embedded sections:
  The whole procedure is performed at room temperature
  1. Deparaffinize and rehydrate tissue section
  2. Block endogenous peroxidase
  3. Wash in PBS
  4. Block with 10% normal goat serum in PBS for 30min. in a humid chamber
  5. Incubate with primary antibody (dilution see datasheet) for 1h in a humid chamber
  6. Wash in PBS
  7. Incubate with secondary antibody (peroxidase-conjugated goat anti mouse IgG+IgM (H+L) minimal-cross reaction to human) for 1h in a humid chamber
  8. Wash in PBS
  9. Incubate with AEC substrate (3-amino-9-ethylcarbazol) for 12min.
  10. Wash in PBS
  11. Counterstain with Mayer’s hemalum