Pdpn Rabbit Polyclonal Antibody

CAT#: AP60017PU-N

Pdpn rabbit polyclonal antibody, Purified

Product Images

Western analysis of anti-Mouse Podoplanin Antibody. Samples were loaded in 15% SDS-polyacrylamide gel under reducing conditions. Lane 1: MWM (kDa); Lane 2: rh sPodoplanin; Lane 3: rm sPodoplanin; Lane 4: rr sPodoplanin.

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: Western blot: 1-5 µg/ml.
  Immunofluorescence/Immunohistochemistry: 10 µg/ml.
• Reactivities: Mouse
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Recombinant Mouse soluble Podoplanin (Gly23-Leu141) derived from E. coli (Cat.-No AR31060PU-N)
• Specificity: This antibody recognizes Mouse Podoplanin. Other species not tested.
• Formulation: PBS
  State: Purified
  State: Lyophilized purified Ig fraction
  Stabilizer: None
  Preservative: None
• Reconstitution Method: Restore in sterile water to a concentration of 0.1-1.0 mg/ml.
• Purification: Protein A Chromatography
• Conjugation: Unconjugated
• Storage: Store lyophilized at 2-8°C for 6 months or at -20°C long term.
  After reconstitution store the antibody undiluted at 2-8°C for one month
  or (in aliquots) at -20°C long term.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: podoplanin
• Database Link:
  Entrez Gene 14726 Mouse
  UniProt ID Q62011
• Background: Podoplanin, also known as glycoprotein 36 (gp36), PA2.26 antigen, T1-alpha (T1A), and aggrus, is a 36 kDa type I transmembrane sialoglycoprotein and member of the Podoplanin family. Podoplanin has three potential splice variants, the longest of which is represented by a 238 amino acid precursor. It contains an undefined signal sequence, a 22 aa transmembrane segment (aa 207-228) and a short cytoplasmic tail (aa 229-238). The cytoplasmic tail contains putative sites for protein kinase C phosphorylation. There are two potential alternate start sites at Met 77 (Swiss Prot #: Q86YL7) and Met 119 (EAW51692) that generate short forms. The 162 aa short form Podoplanin precursor shares 47% aa identity with mouse Podoplanin. Podoplanin is expressed on glomerular epithelial cells (podocytes), type I lung alveolar cells, lymphatic endothelial cells, and numerous tumors, including colorectal tumors, squamous cell carcinomas, testicular seminoma, and brain tumors. One study shows high expression of Podoplanin mRNA in placenta, lung, skeletal muscle, and heart, and weaker levels in brain, kidney, and liver. Podoplanin is the ligand for C-type lectin-like receptor 2 (CLEC-2). Their association is dependent on sialic acid on O-glycans of Podoplanin. Through its association with CLEC-2, Podoplanin induces platelet aggregation and tumor metastasis. Podoplanin is also necessary for lymphatic vessel formation, normal lung cell proliferation and alveolus formation at birth.
• Synonyms: Glycoprotein 36, PA2.26 antigen, T1-alpha, Aggrus, PDPN, GP36, PSEC0003, PSEC0025