SPARCL1 (Center) Rabbit Polyclonal Antibody

CAT#: AP54000PU-N

SPARCL1 (Center) rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot analysis in NCI-H460 cell line lysates (35ug/lane) using SPARCL1 / Hevin antibody. This demonstrates the SPARCL1 antibody detected the SPARCL1 protein (arrow).

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  Immunohistochemistry analysis in human brain tissue (Formalin-fixed, Paraffin-embedded) using SPARCL1 / Hevin antibody., followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the SPARCL1 antibody for IHC; Clinical relevance has not been evaluated.

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution:

  Immunohistochemistry on Paraffin sections: 1/50-1/100.
  Western blot: 1/100-1/500.
  Enzyme immunoassay: 1/1000.

• Reactivities: Human
• Host: Rabbit
• Isotype: Ig
• Clonality: Polyclonal
• Immunogen: Synthetic peptide - KLH conjugated - corresponding to the central region (between 215-244aa) of human SPARCL1
• Specificity: This antibody recognizes SPARCL1.
• Formulation: PBS with 0.09% (W/V) Sodium azide
  State: Aff - Purified
  State: Liquid purified Ig fraction
• Concentration: lot specific
• Purification: Purified through a Protein A column followed by peptide affinity purification
• Conjugation: Unconjugated
• Storage:

  Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.

• Stability: Shelf life: one year from despatch.

• Gene Name: Homo sapiens SPARC like 1 (SPARCL1), transcript variant 1
• Database Link:
  Entrez Gene 8404 Human
  UniProt ID Q14515
• Synonyms: SPARC-Like Protein 1, MAST9, MAST 9, PIG33
• Note: Molecular Weight: 75208 Da

Reference Data

• Protein Families: Druggable Genome, Secreted Protein