Calcium independent Phospholipase A2 (PLA2G6) (Center) Rabbit Polyclonal Antibody

CAT#: AP53326PU-N

Calcium independent Phospholipase A2 (PLA2G6) (Center) rabbit polyclonal antibody, Purified

Product Images

Western blot analysis of PLA2G6 (arrow) in HepG2 cell line lysates (35ug/lane) using PLA2G6 antibody.

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  Immunohistochemistry analysis in testis carcinoma (Formalin-fixed, Paraffin-embedded) using PLA2G6 antibody., followed by peroxidase conjugated secondary antibody and DAB staining. This data demonstrates the use of the PLA2G6 antibody for IHC. Clinical relevance has not been evaluated.

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  Confocal immunofluorescent analysis with HepG2 cell using PLA2G6 antibody., followed by Alexa Fluor® 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).

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  Flow cytometric analysis of HepG2 cells (right histogram) compared to a negative control cell (left histogram) using PLA2G6 antibody., followed by FITC-conjugated goat-anti-rabbit secondary antibodies.

Specifications

Product Data

• Applications: FC, IF, IHC, WB
• Recommended Dilution:

  Flow cytometry: 1/10-1/50.
  Immunofluorescence: 1/10-1/50.
  Immunohistochemistry on Paraffin sections: 1/50-1/100.
  Western blot: 1/50-1/100.

• Reactivities: Human
• Host: Rabbit
• Isotype: Ig
• Clonality: Polyclonal
• Immunogen: Synthetic peptide - KLH conjugated - corresponding to the central region (between 558-588aa) of human PLA2G6.
• Specificity: This antibody recognizes PLA2G6.
• Formulation: PBS with 0.09% (W/V) Sodium azide
  State: Purified
  State: Liquid purified Ig fraction
• Concentration: lot specific
• Purification: Prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS
• Conjugation: Unconjugated
• Storage:

  Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.

• Stability: Shelf life: one year from despatch.

• Gene Name: phospholipase A2 group VI
• Database Link:
  Entrez Gene 8398 Human
  UniProt ID O60733
• Background: The protein encoded by the PLA2G6 gene is an A2 phospholipase, a class of enzyme that catalyzes the release of fatty acids from phospholipids. The encoded protein may play a role in phospholipid remodelling, arachidonic acid release, leukotriene and prostaglandin synthesis, fas-mediated apoptosis, and transmembrane ion flux in glucose-stimulated B-cells.
• Synonyms: IPLA2, Cal-PLA2, GVI PLA2, Group VI phospholipase A2, PARK14
• Note: Molecular Weight: 89903 Da

Reference Data

• Protein Pathways: alpha-Linolenic acid metabolism, Arachidonic acid metabolism, Ether lipid metabolism, Fc epsilon RI signaling pathway, Fc gamma R-mediated phagocytosis, Glycerophospholipid metabolism, GnRH signaling pathway, Linoleic acid metabolism, Long-term depression, MAPK signaling pathway, Metabolic pathways, Vascular smooth muscle contraction, VEGF signaling pathway