RNF70 (PJA1) (N-term) Rabbit Polyclonal Antibody

CAT#: AP53313PU-N

RNF70 (PJA1) (N-term) rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot analysis of PJA1 / RNF70 Antibody (N-term) in Hela cell line lysates (35ug/lane). This demonstrates the PJA1 antibody detected the PJA1 protein (arrow).

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  Immunohistochemistry analysis in formalin fixed and paraffin embedded human cerebellum tissue reacted with PJA1 / RNF70 Antibody (N-term), which was peroxidase conjugated to the secondary antibody and followed by DAB staining. This data demonstrates the use of PJA1 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: ELISA: 1/1000.
  Western Blot: 1/100-1/500.
  Immunohistochemistry on Paraffin Sections: 1/10-1/50.
• Reactivities: Human
• Host: Rabbit
• Isotype: Ig
• Clonality: Polyclonal
• Immunogen: KLH conjugated synthetic peptide between 154-183 amino acids from the N-terminal region of human PJA / RNF70
• Specificity: This antibody recognizes Human PJA1 / RNF70 (N-term).
• Formulation: PBS containing 0.09% (W/V) Sodium Azide as preservative
  State: Aff - Purified
  State: Liquid purified Ig fraction
• Concentration: lot specific
• Purification: Protein A column, followed by peptide affinity purification
• Conjugation: Unconjugated
• Storage:

  Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.

• Stability: Shelf life: one year from despatch.

• Gene Name: praja ring finger ubiquitin ligase 1
• Database Link:
  Entrez Gene 64219 Human
  UniProt ID Q8NG27
• Background: This gene encodes an enzyme that has E2-dependent E3 ubiquitin-protein ligase activity. This enzyme belongs to a class of ubiquitin ligases that include a RING finger motif, and it can interact with the E2 ubiquitin-conjugating enzyme UbcH5B. This gene is located in an area of chromosome X where several X-linked mental retardation disorders have been associated, and it has also been found as part of a contiguous gene deletion associated with craniofrontonasal syndrome, though a direct link to any disorder has yet to be demonstrated. Alternative splicing results in multiple transcript variants.
• Synonyms: Praja1, Praja-1, RING finger protein 70
• Note: Molecular Weight: 71002 Da

Reference Data

• Protein Families: Druggable Genome