Myeloperoxidase (MPO) (N-term) Rabbit Polyclonal Antibody

CAT#: AP52788PU-N

Myeloperoxidase (MPO) (N-term) rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot analysis in HL-60 cell line lysates (35ug/lane) using Myeloperoxidase antibody. (C-term). This demonstrates this antibody detected the Myeloperoxidase protein (arrow).

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  Flow cytometric analysis of HL-60 cells (right histogram) compared to a negative control cell (left histogram) using Myeloperoxidase antibody. (C-term), followed by FITC-conjugated goat-anti-rabbit secondary antibodies.

Specifications

Product Data

• Applications: FC, WB
• Recommended Dilution: Flow cytometry: 1/10-1/50.
  Western blot: 1/100-1/500.
  Enzyme immunoassay: 1/1000.
• Reactivities: Human
• Host: Rabbit
• Isotype: Ig
• Clonality: Polyclonal
• Immunogen: Synthetic peptide - KLH conjugated - corresponding to the N-terminal region (between 66-97aa) of human Myeloperoxidase
• Specificity: This antibody recognizes human Myeloperoxidase at N-term.
• Formulation: PBS with 0.09% (W/V) Sodium azide
  State: Aff - Purified
  State: Liquid purified Ig fraction
• Concentration: lot specific
• Purification: Purified through a Protein A column followed by peptide affinity purification
• Conjugation: Unconjugated
• Storage:

  Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.

• Stability: Shelf life: one year from despatch.

• Gene Name: myeloperoxidase
• Database Link:
  Entrez Gene 4353 Human
  UniProt ID P05164
• Background: Myeloperoxidase (MPO) is a heme protein synthesized during myeloid differentiation that constitutes the major component of neutrophil azurophilic granules. Produced as a single chain precursor, myeloperoxidase is subsequently cleaved into a light and heavy chain. The mature myeloperoxidase is a tetramer composed of 2 light chains and 2 heavy chains. This enzyme produces hypohalous acids central to the microbicidal activity of netrophils.
• Synonyms: MPO
• Note: Molecular Weight: 83869 Da

Reference Data

• Protein Families: Druggable Genome