CRSP9 (MED7) (Center) Rabbit Polyclonal Antibody

CAT#: AP52659PU-N

CRSP9 (MED7) (Center) rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot analysis of MED7 Antibody (Center) in Hela cell line lysates (35ug/lane). This demonstrates the MED7 antibody detected the MED7 protein (arrow).

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  Flow cytometric analysis of Hela cells using MED7 Antibody (Center) (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Specifications

Product Data

• Applications: FC, WB
• Recommended Dilution: ELISA: 1/1000.
  Western Blot: 1/100-1/500.
  Flow Cytometry: 1/10-1/50.
• Reactivities: Human
• Host: Rabbit
• Isotype: Ig
• Clonality: Polyclonal
• Immunogen: KLH conjugated synthetic peptide between 90-119 amino acids from the Central region of human MED7
• Specificity: This antibody recognizes Human MED7 (Center).
• Formulation: PBS containing 0.09% (W/V) Sodium Azide as preservative
  State: Aff - Purified
  State: Liquid purified Ig fraction
• Concentration: lot specific
• Purification: Protein A column, followed by peptide affinity purification
• Conjugation: Unconjugated
• Storage:

  Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.

• Stability: Shelf life: one year from despatch.

• Gene Name: mediator complex subunit 7
• Database Link:
  Entrez Gene 9443 Human
  UniProt ID O43513
• Background: The activation of gene transcription is a multistep process that is triggered by factors that recognize transcriptional enhancer sites in DNA. These factors work with co-activators to direct transcriptional initiation by the RNA polymerase II apparatus. The protein encoded by this gene is a subunit of the CRSP (cofactor required for SP1 activation) complex, which, along with TFIID, is required for efficient activation by SP1. This protein is also a component of other multisubunit complexes e.g. thyroid hormone receptor-(TR-) associated proteins which interact with TR and facilitate TR function on DNA templates in conjunction with initiation factors and cofactors. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq].
• Synonyms: ARC34, CRSP9, Mediator complex subunit 7
• Note: Molecular Weight: 27245 Da

Reference Data

• Protein Families: Druggable Genome, Transcription Factors