Lysyl tRNA synthetase (KARS) (N-term) Rabbit Polyclonal Antibody

CAT#: AP52290PU-N

Lysyl tRNA synthetase (KARS) (N-term) rabbit polyclonal antibody, Purified

Product Images

Western blot analysis of KARS Antibody (N-term) in MCF-7 cell line lysates (35ug/lane). This demonstrates the KARS antibody detected the KARS protein (arrow).

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  Western blot analysis of KARS (arrow) using KARS Antibody (N-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the KARS gene.

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  Flow cytometric analysis of 293 cells using KARS Antibody (N-term) (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Specifications

Product Data

• Applications: FC, WB
• Recommended Dilution: ELISA: 1/1000.
  Western Blot: 1/100-1/500.
  Flow Cytometry: 1/10-1/50.
• Reactivities: Human
• Host: Rabbit
• Isotype: Ig
• Clonality: Polyclonal
• Immunogen: KLH conjugated synthetic peptide between 77-105 amino acids from the N-terminal region of human KARS
• Specificity: This antibody recognizes Human KARS (N-term).
• Formulation: PBS containing 0.09% (W/V) Sodium Azide as preservative
  State: Purified
  State: Liquid purified Ig fraction
• Concentration: lot specific
• Purification: Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS
• Conjugation: Unconjugated
• Storage:

  Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.

• Stability: Shelf life: one year from despatch.

• Gene Name: lysyl-tRNA synthetase
• Database Link:
  Entrez Gene 3735 Human
  UniProt ID Q15046
• Background: Aminoacyl-tRNA synthetases are a class of enzymes that charge tRNAs with their cognate amino acids. Lysyl-tRNA synthetase is a homodimer localized to the cytoplasm which belongs to the class II family of tRNA synthetases. It has been shown to be a target of autoantibodies in the human autoimmune diseases, polymyositis or dermatomyositis. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
• Synonyms: Lysyl-tRNA synthetase, KIAA0070, LysRS, Lysine-tRNA ligase
• Note: Molecular Weight: 68048 Da

Reference Data

• Protein Pathways: Aminoacyl-tRNA biosynthesis