INPP5B (C-term) Rabbit Polyclonal Antibody

CAT#: AP52214PU-N

INPP5B (C-term) rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot analysis of INPP5B / 5PTase 2 Antibody (C-term) in CEM cell line lysates (35ug/lane). This demonstrates the INPP5B antibody detected the INPP5B protein (arrow).

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  Immunohistochemistry analysis in formalin fixed and paraffin embedded human skeletal muscle reacted with INPP5B / 5PTase 2 Antibody (C-term) followed by peroxidase conjugation of the secondary antibody and DAB staining.

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  Flow cytometric analysis of CEM cells using INPP5B / 5PTase 2 Antibody (C-term) (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Specifications

Product Data

• Applications: FC, IHC, WB
• Recommended Dilution:

  ELISA: 1/1000.
  Western Blot: 1/100-1/500.
  Flow Cytometry: 1/10-1/50.
  Immunohistochemistry on Paraffin Sections: 1/50-1/100.

• Reactivities: Human
• Host: Rabbit
• Isotype: Ig
• Clonality: Polyclonal
• Immunogen: KLH conjugated synthetic peptide between 957-987 amino acids from the C-terminal region of Human INPP5B / 5PTase 2.
• Specificity: This antibody recognizes Human INPP5B / 5PTase 2 (C-term).
• Formulation: PBS containing 0.09% (W/V) Sodium Azide as preservative
  State: Aff - Purified
  State: Liquid purified Ig fraction
• Concentration: lot specific
• Purification: Protein A column, followed by peptide affinity purification
• Conjugation: Unconjugated
• Storage:

  Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.

• Stability: Shelf life: one year from despatch.

• Gene Name: inositol polyphosphate-5-phosphatase B
• Database Link:
  Entrez Gene 3633 Human
  UniProt ID P32019
• Background: Cellular calcium signaling is controlled by the production of inositol phosphates (IPs) by phospholipase C in response to extracellular signals. The IP signaling molecules are inactivated by a family of inositol polyphosphate-5-phosphatases (5-phosphatases). INPP5B encodes the type II 5-phosphatase. The protein is localized to the cytosol and mitochondria, and associates with membranes through an isoprenyl modification near the C-terminus. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some of these variants has not been determined. [provided by RefSeq].
• Synonyms: 5PTase; MGC65156; MGC71303
• Note: Molecular Weight: 112852 Da

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Inositol phosphate metabolism, Metabolic pathways, Phosphatidylinositol signaling system