Gephyrin (GPHN) (Center) Rabbit Polyclonal Antibody

CAT#: AP51909PU-N

Gephyrin (GPHN) (Center) rabbit polyclonal antibody, Aff - Purified

Product Images

GPHN Antibody (Center) western blot analysis in MCF-7, Hela, NCI-H460, HepG2 cell line and mouse liver tissue lysates (35ug/lane).This demonstrates the GPHN antibody detected the GPHN protein (arrow).

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  GPHN Antibody (Center) IHC analysis in formalin fixed and paraffin embedded brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the GPHN Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.

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  GPHN Antibody (Center) flow cytometric analysis of MCF-7 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Specifications

Product Data

• Applications: FC, IHC, WB
• Recommended Dilution: ELISA: 1/1000.
  Western Blot: 1/100 - 1/500.
  Immunohistochemistry on paraffin sections: 1/50 - 1/100.
  Flow Cytometry: 1/10 - 1/50.
• Reactivities: Human, Mouse
• Host: Rabbit
• Isotype: Ig
• Clonality: Polyclonal
• Immunogen: KLH conjugated synthetic peptide between 444~474 amino acids from the Central region of human GPHN
• Specificity: This antibody reacts to GPHN.
• Formulation: PBS
  State: Aff - Purified
  State: Liquid purified Ig fraction
  Preservative: 0.09% (W/V) sodium azide
• Concentration: lot specific
• Purification: Affinity chromatography on Protein A
• Conjugation: Unconjugated
• Storage:

  Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.

• Stability: Shelf life: one year from despatch.

• Predicted Protein Size: 79748 Da
• Gene Name: gephyrin
• Database Link:
  Entrez Gene 268566 MouseEntrez Gene 10243 Human
  UniProt ID Q9NQX3
• Background: GPHN encodes a neuronal assembly protein that anchors inhibitory neurotransmitter receptors to the postsynaptic cytoskeleton via high affinity binding to a receptor subunit domain and tubulin dimers. In nonneuronal tissues, the encoded protein is also required for molybdenum cofactor biosynthesis. Mutations in this gene may be associated with the neurological condition hyperplexia and also lead to molybdenum cofactor deficiency.
• Synonyms: GPHN, GPH, KIAA1385

Reference Data

• Protein Families: Druggable Genome