EFHB (N-term) Rabbit Polyclonal Antibody

CAT#: AP51376PU-N

EFHB (N-term) rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot analysis in MCF-7 cell line lysates (35ug/lane) using EFHB antibody. (N-term). This demonstrates this antibody detected the EFHB protein (arrow).

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  Immunohistochemistry analysis in human kidney tissue (Formalin-fixed, Paraffin-embedded) using EFHB antibody. (N-term), followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of this antibody for IHC. Clinical relevance has not been evaluated.

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  Flow cytometric analysis of MCF-7 cells (right histogram) compared to a negative control cell left histogram) using EFHB antibody. (N-term), followed by FITC-conjugated goat-anti-rabbit secondary antibodies.

Specifications

Product Data

• Applications: FC, IHC, WB
• Recommended Dilution:

  Flow cytometry: 1/10-1/50.
  Immunohistochemistry on Paraffin sections: 1/50-1/100.
  Western blot: 1/100-1/500.
  Enzyme immunoassay: 1/1000.

• Reactivities: Human
• Host: Rabbit
• Isotype: Ig
• Clonality: Polyclonal
• Immunogen: Synthetic peptide - KLH conjugated - corresponding to the N-terminal region (between 1-30aa) of human EFHB.
• Specificity: This antibody recognizes human EFHB at N-term.
• Formulation: PBS with 0.09% (W/V) Sodium azide
  State: Aff - Purified
  State: Liquid purified Ig fraction
• Concentration: lot specific
• Purification: Purified through a Protein A column followed by peptide affinity purification
• Conjugation: Unconjugated
• Storage:

  Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.

• Stability: Shelf life: one year from despatch.

• Gene Name: EF-hand domain family member B
• Database Link:
  Entrez Gene 151651 Human
  UniProt ID Q8N7U6
• Background: EFHB contains two EF-hand domains. There are three named isoforms.
• Synonyms: FLJ25200
• Note: Molecular Weight: 93802 Da