COX6A1 (Center) Rabbit Polyclonal Antibody

CAT#: AP51033PU-N

COX6A1 (Center) rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot analysis of COX6A1 Antibody (Center) in MDA-MB435 cell line lysates (35ug/lane). COX6A1 (arrow) was detected using the purified Pab.

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  Western blot analysis of COX6A1 (arrow) using rabbit polyclonal COX6A1 Antibody (Center). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the COX6A1 gene.

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  Formalin-fixed and paraffin-embedded human testis tissue reacted with COX6A1 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

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  COX6A1 Antibody (Center) FC analysis of ZR-75-1 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Specifications

Product Data

• Applications: FC, IHC, WB
• Recommended Dilution: Western blot: 1/1000.
  Immunohistochemistry on paraffin sections: 1/50 - 1/100.
  Flow cytometry: 1/10 - 1/50.
• Reactivities: Human
• Host: Rabbit
• Isotype: Ig
• Clonality: Polyclonal
• Immunogen: KLH conjugated synthetic peptide between 50 - 78 amino acids from the Center region of human COX6A1
• Specificity: This antibody reacts to COX6A1.
• Formulation: PBS
  State: Aff - Purified
  State: Liquid purified Ig fraction
  Preservative: 0.09% (W/V) sodium azide
• Concentration: lot specific
• Purification: Affinity chromatography on Protein A followed by peptide affinity purification.
• Conjugation: Unconjugated
• Storage:

  Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.

• Stability: Shelf life: one year from despatch.

• Predicted Protein Size: 12155 Da
• Gene Name: cytochrome c oxidase subunit 6A1
• Database Link:
  Entrez Gene 1337 Human
  UniProt ID P12074
• Background: This protein is one of the nuclear-coded polypeptide chains of cytochrome c oxidase, the terminal oxidase in mitochondrial electron transport.
• Synonyms: Cytochrome c oxidase subunit 6A1, COX6A1, COX6AL, Mitochondria Complex IV

Reference Data

• Protein Families: Transmembrane
• Protein Pathways: Alzheimer's disease, Cardiac muscle contraction, Huntington's disease, Metabolic pathways, Oxidative phosphorylation, Parkinson's disease