Cyclin T1 (CCNT1) (Center) Rabbit Polyclonal Antibody

CAT#: AP50822PU-N

Cyclin T1 (CCNT1) (Center) rabbit polyclonal antibody, Aff - Purified

Product Images

CCNT1 Antibody (Center) western blot analysis in mouse testis tissue lysates (35ug/lane).This demonstrates the CCNT1 antibody detected the CCNT1 protein (arrow).

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  CCNT1 Antibody (Center) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the CCNT1 antibody detected the CCNT1 protein (arrow).

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  CCNT1 Antibody (Center) flow cytometric analysis of MCF-7 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Specifications

Product Data

• Applications: FC, IHC, WB
• Recommended Dilution: ELISA: 1/1000.
  Western blot: 1/100 - 1/500.
  Immunohistochemistry on paraffin sections: 1/50 - 1/100.
  Flow Cytometry: 1/10 - 1/50.
• Reactivities: Human, Mouse
• Host: Rabbit
• Isotype: Ig
• Clonality: Polyclonal
• Immunogen: KLH conjugated synthetic peptide between 256-285 amino acids from the Central region of human CCNT1
• Specificity: This antibody reacts to CCNT1.
• Formulation: PBS
  State: Aff - Purified
  State: Liquid purified Ig fraction
  Preservative: 0.09% (W/V) sodium azide
• Concentration: lot specific
• Purification: Affinity chromatography on Protein A
• Conjugation: Unconjugated
• Storage:

  Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.

• Stability: Shelf life: one year from despatch.

• Predicted Protein Size: 80685 Da
• Gene Name: cyclin T1
• Database Link:
  Entrez Gene 12455 MouseEntrez Gene 904 Human
  UniProt ID O60563
• Background: CCNT1 belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin tightly associates with CDK9 kinase, and was found to be a major subunit of the transcription elongation factor p-TEFb. The kinase complex containing this cyclin and the elongation factor can interact with, and act as a cofactor of human immunodeficiency virus type 1 (HIV-1) Tat protein, and was shown to be both necessary and sufficient for full activation of viral transcription. This cyclin and its kinase partner were also found to be involved in the phosphorylation and regulation of the carboxy-terminal domain (CTD) of the largest RNA polymerase II subunit.
• Synonyms: Cyclin-T1, Cyclin-T, CycT1, CCNT1

Reference Data

• Protein Families: Druggable Genome, Transcription Factors