Acyl coenzyme A Thioesterase 8 (ACOT8) (C-term) Rabbit Polyclonal Antibody

CAT#: AP50053PU-N

Acyl coenzyme A Thioesterase 8 (ACOT8) (C-term) rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot analysis of ACOT8 Antibody (C-term) in mouse liver tissue lysates (35 ug/lane). ACOT8 (arrow) was detected using the purified Pab.

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  Formalin-fixed and paraffin-embedded human Prostate carcinoma reacted with ACOT8 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

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  ACOT8 Antibody (C-term) flow cytometric analysis of MDA-MB435 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Specifications

Product Data

• Applications: FC, IHC, WB
• Recommended Dilution: ELISA: 1/1000.
  Western blotting: 1/100 - 1/500.
  Immunohistochemistry: 1/50 - 1/100.
  Flow cytometry: 1/10 - 1/50.
• Reactivities: Human, Mouse
• Host: Rabbit
• Isotype: Ig
• Clonality: Polyclonal
• Immunogen: KLH conjugated synthetic peptide between 290~319 amino acids from the C-terminal region of human ACOT8
• Specificity: This antibody reacts to ACOT8.
• Formulation: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column; followed by peptide affinity purification.
  State: Aff - Purified
  State: Liquid purified Ig fraction
• Concentration: lot specific
• Purification: Affinity chromatography on Protein A
• Conjugation: Unconjugated
• Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: acyl-CoA thioesterase 8
• Database Link:
  Entrez Gene 170789 MouseEntrez Gene 10005 Human
  UniProt ID O14734
• Background: Acyl-CoA thioesterases are a group of enzymes that catalyze the hydrolysis of acyl-CoAs to the free fatty acid and coenzyme A (CoASH), providing the potential to regulate intracellular levels of acyl-CoAs, free fatty acids and CoASH. It may mediate Nef-induced down-regulation of CD4. It may be involved in the metabolic regulation of peroxisome proliferation.
• Synonyms: ACTEIII, PTE1, PTE2, Acyl-CoA thioesterase 8, PTE-2
• Note: Molecular Weight: 35914 Da