ZIC3 (178-189) Goat Polyclonal Antibody

CAT#: AP33292PU-N

ZIC3 (178-189) goat polyclonal antibody, Aff - Purified

Product Images

Staining of paraffin embedded Human Prostate using ZIC3 Antibody at 5 ug/ml. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Specifications

Product Data

• Applications: ELISA, IHC
• Recommended Dilution: Peptide ELISA: 1/64000 (Detection Limit).
  Western blot: Preliminary experiments gave an approx 70kDa band in Human, Mouse and Rat Brain lysates after 1µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 50.6kDa according to NP_003404.1. The 70kDa band was successfully blocked by incubation with the immunizing peptide.
  Immunohistochemistry on Paraffin Sections: 5-10 µg/ml. In paraffin embedded Human Prostate shows nuclear staining in select secretory cells.
• Reactivities: Bovine, Human, Mouse, Porcine, Rat
• Host: Goat
• Clonality: Polyclonal
• Immunogen: Peptide with sequence from the internal region of the protein sequence according to NP_003404.1.
• Specificity: This antibody detects ZIC3 (178-189) and is expected NOT to cross-react with other ZIC proteins.
• Formulation: Tris saline, pH~7.3
  State: Aff - Purified
  State: Liquid purified Ig fraction
  Stabilizer: 0.5% BSA
  Preservative: 0.02% Sodium Azide
• Concentration: lot specific
• Purification: Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: Zic family member 3
• Database Link:
  Entrez Gene 7547 Human
  UniProt ID O60481
• Synonyms: HTX; HTX1; ZNF203