PIM2 (23-36) Goat Polyclonal Antibody

CAT#: AP32855PU-N

PIM2 (23-36) goat polyclonal antibody, Aff - Purified

Product Images

HEK293 lysate (10ug protein in RIPA buffer) overexpressing Human PIM2 with C-terminal MYC tag probed with (0.1ug/ml) in Lane A and probed with anti-MYC Tag (1/1000) in lane C. Mock-transfected HEK293 probed with (0.1mg/ml) in Lane B. Primary incubations were for 1 hour. Detected by chemiluminescence.

Specifications

Product Data

• Applications: ELISA, IHC, WB
• Recommended Dilution: Peptide ELISA: 1/64000 (Detection Limit).
  Western blot: 0.5-1 μg/ml. In transfected HEK293 transiently expressing Human PIM2 (myc and DYKDDDDK tagged), a band of approx. 38kDa is observed. No bands are observed in mock-transfected HEK293 and the same band is observed using anti-myc antibody.
  Immunohistochemistry on Paraffin Sections: 5µg/ml. Paraffin embedded Human Heart.
• Reactivities: Bovine, Human, Mouse, Rat
• Host: Goat
• Clonality: Polyclonal
• Immunogen: Peptide with sequence from the internal region (near N Terminus) of the protein sequence according to NP_006866.2.
• Specificity: This antibody recognizes PIM2 (23-36).
• Formulation: Tris saline, pH~7.3
  State: Aff - Purified
  State: Liquid purified IgG fraction
  Stabilizer: 0.5% BSA
  Preservative: 0.02% Sodium Azide
• Concentration: lot specific
• Purification: Ammonium Sulphate precipitation followed by Antigen Affinity Chromatography using the immunizing peptide
• Conjugation: Unconjugated
• Storage:

  Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.

• Stability: Shelf life: one year from despatch.

• Gene Name: Pim-2 proto-oncogene, serine/threonine kinase
• Database Link:
  Entrez Gene 11040 Human
  UniProt ID Q9P1W9
• Synonyms: Pim-2, Pim-2h