LYZ Rabbit Polyclonal Antibody

CAT#: AP32827BT-N

LYZ rabbit polyclonal antibody, Biotin

Specifications

Product Data

• Applications: ELISA, IF, IHC, WB
• Recommended Dilution: ELISA.
  Immunocytochemistry.
  Immunohistochemistry on Frozen Sections.
  Western blotting.
  Recommended Working Dilutions:
  histochemical and cytochemical Use: 1/10-1/50.
• Reactivities: Human
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Highly purified lysozyme is isolated from pooled milk.
  Freund's complete adjuvant is used in the first step of the immunization procedure.
• Specificity: The reactivity of the antiserum is restricted to Lysozyme. In immunoelectrophoresis and radial immunodiffusion (Ouchterlony), using various antiserum concentrations against normal human milk and tears a single precipitin line is obtained which shows a reaction of identity with the precipitin line obtained with purified lysozyme. No reaction is obtained with any other plasma protein component or serum.
  Cross-reactivity: The antiserum does not cross-react with any other component of human plasma. Inter-species cross-reactivity is a normal feature of antibodies to plasma proteins since they frequently share antigenic determinants. Cross-reactivity of this antiserum has not been tested in detail.
• Formulation: PBS, pH 7.2
  Label: Biotin
  State: Lyophilized purified hyperimmune IgG fraction
  Stabilizer: None
  Preservative: None
  Label: Fluorescein isothiocyanate isomer 1
• Reconstitution Method: Restore by by adding 1 ml sterile di stilled water
• Purification: Hyperimmune antisera with strong precipitating activity are selected for fractionation by salt precipitation and purification of the IgG fraction by DEAE-chromatography
• Conjugation: Biotin
• Storage: Store lyophilized at 2-8°C for 6 months or at -20°C long term.
  After reconstitution store the antibody undiluted at 2-8°C for one month
  or (in aliquots) at -20°C long term.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Database Link:
  Entrez Gene 396218 Chicken
  UniProt ID P00698
• Background: Lysozyme, a bacteriolytic enzyme, has a molecular weight of about 15,000. Its concentration in plasma is low; in milk the concentration is reported to be between 5 and 220 mg/ml. Also tears contain a considerable amount of lysozyme.
• Synonyms: LYZ, LZM