HOXA9 (49-60) Goat Polyclonal Antibody

CAT#: AP32715PU-N

HOXA9 (49-60) goat polyclonal antibody, Aff - Purified

Product Images

Staining of Moue Spleen lysate using HOXA9 Antibody at 2 ug/ml (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Specifications

Product Data

• Applications: WB
• Recommended Dilution: Peptide ELISA: 1/8000 (Detection Limit).
  Western blot: 1-3 µg/ml. Approx 36kDa band observed in Mouse Spleen lysates (calculated MW of 29.9kDa according to NP_034586.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Quéré et al, Blood. 2011 Jun 2;117(22):5918-30. PMID: 21471525). An additional band of unknown identity was also consistently observed at 20kDa. This band was successfully blocked by incubation with the immunizing peptide.
• Reactivities: Bovine, Canine, Human, Mouse, Rat
• Host: Goat
• Clonality: Polyclonal
• Immunogen: Peptide with sequence from the internal region of the protein sequence according to NP_689952.1
• Specificity: This antibody recognizes HOXA9 (49-60).
• Formulation: Tris saline, pH~7.3
  State: Aff - Purified
  State: Liquid purified IgG fraction
  Stabilizer: 0.5% BSA
  Preservative: 0.02% Sodium Azide
• Concentration: lot specific
• Purification: Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide
• Conjugation: Unconjugated
• Storage:

  Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.

• Stability: Shelf life: one year from despatch.

• Gene Name: homeobox A9
• Database Link:
  Entrez Gene 3205 Human
  UniProt ID P31269
• Synonyms: Homeobox protein Hox-A9, Hox-1G