EAAT1 (SLC1A3) (197-208) Goat Polyclonal Antibody

CAT#: AP32158PU-N

EAAT1 (SLC1A3) (197-208) goat polyclonal antibody, Aff - Purified

Product Images

Staining of Human Frontal Cortex lysate using SLC1A3 / EAAT1 antibody at 0.1 ug/ml (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Specifications

Product Data

• Applications: WB
• Recommended Dilution: Peptide ELISA: 1/128000 (Detection Limit).
  Western blot: 0.1-0.3 μg/ml. Approx 60kDa band observed in Human Brain (Frontal Cortex) lysates (calculated MW of 59.6kDa according to NP_004163.3).
  An additional band of unknown identity was also consistently observed at 22kDa. This band was successfully blocked by incubation with the immunizing peptide.
• Reactivities: Human, Rat
• Host: Goat
• Clonality: Polyclonal
• Immunogen: Peptide with sequence from the internal region of the protein sequence according to NP_004163.3; NP_001160167.1.
• Specificity: This antibody is expected to recognize isoform 1 (NP_004163.3) and isoform 2 (NP_001160167.1) of EAAT1 / GLAST1.
• Formulation: Tris saline, pH~7.3
  State: Aff - Purified
  State: Liquid purified IgG fraction
  Stabilizer: 0.5% BSA
  Preservative: 0.02% Sodium Azide
• Concentration: lot specific
• Purification: Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide
• Conjugation: Unconjugated
• Storage:

  Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.

• Stability: Shelf life: one year from despatch.

• Gene Name: solute carrier family 1 member 3
• Database Link:
  Entrez Gene 6507 Human
  UniProt ID P43003
• Synonyms: GLAST, GLAST1, GLAST-1