Cntn4 (160-172) Goat Polyclonal Antibody

CAT#: AP32063PU-N

Cntn4 (160-172) goat polyclonal antibody, Aff - Purified

Product Images

Contactin-4 / Big-2 Antibody (0.5 ug/ml) staining of Mouse Olfactory bulb (Lanes 1 and 3) and Cerebral cortex (Lanes 2 and 4), comparing wildtype (lanes 3 and 4) with KO mice (Lanes 1 and 2). The last lane contains a lysate of HEK293 overexpressing Mouse Cntn4 (lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Data obtained from Gerrald Lodewijk and Peter Burbach, Rudolf Magnus Institute, Utrecht, Netherlands.

Specifications

Product Data

• Applications: ELISA, WB
• Recommended Dilution:

  Peptide ELISA: 1/128000 (Detection Limit).
  Western blot: 0.5-1.5 μg/ml. Approx 150kDa band observed in Mouse Brain lysates and not in the same lysates from the KO mice (calculated MW of 113kDa according to NP_001103219.1).

• Reactivities: Canine, Human, Mouse, Rat
• Host: Goat
• Clonality: Polyclonal
• Immunogen: Peptide with sequence from the internal region of the protein sequence according to NP_001103219.1; NP_766592.2.
• Specificity:
  This antibody is expected to recognize both reported isoforms of Contactin 4 (NP_001103219.1; NP_766592.2).
  Reported variants represent identical protein: NP_766592.2, NP_001103221.1
• Formulation: Tris saline, pH~7.3
  State: Aff - Purified
  State: Liquid purified Ig fraction
  Stabilizer: 0.5% BSA
  Preservative: 0.02% Sodium Azide
• Concentration: lot specific
• Purification: Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide
• Conjugation: Unconjugated
• Storage:

  Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.

• Stability: Shelf life: one year from despatch.

• Gene Name: contactin 4
• Database Link:
  Entrez Gene 269784 Mouse
  UniProt ID Q69Z26
• Synonyms: CNTN4, BIG-2, BIG2