beta III Tubulin (TUBB3) Goat Polyclonal Antibody

CAT#: AP32033PU-N

beta III Tubulin (TUBB3) goat polyclonal antibody, Aff - Purified

Product Images

TUBB3 Antibody staining of HepG2 lysate at 2 ug/ml (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Specifications

Product Data

• Applications: WB
• Recommended Dilution:

  Peptide ELISA: 1/1000 (Detection Limit).
  Western blot: 1-3 μg/ml. Detects a band of Approx 52kDa band observed in lysates of cell line HepG2.

• Reactivities: Bovine, Human, Mouse, Porcine, Rat
• Host: Goat
• Clonality: Polyclonal
• Immunogen: Synthetic peptide from the internal region (near N terminus) of Human TUBB3 (NP_006077.2).
• Specificity: This antibody is expected to recognize isoform 1 (NP_006077.2) only.
• Formulation:
  Tris saline, pH~7.3
  
  State: Aff - Purified
  State: Liquid purified Ig fraction
  Stabilizer: 0.5% BSA
  Preservative: 0.02% Sodium Azide
• Concentration: lot specific
• Purification: Ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated
• Storage:

  Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.

• Stability: Shelf life: one year from despatch.

• Predicted Protein Size: 50.4 kDa (NP_006077.2).
• Database Link:
  Entrez Gene 10381 Human
  UniProt ID Q13509
• Synonyms: Tubulin beta-3 chain, Tubulin beta-III, Tubulin beta-4