XAGE1 (XAGE1E) (Isoform 1d) Goat Polyclonal Antibody

CAT#: AP31980PU-N

XAGE1 (XAGE1E) (Isoform 1d) goat polyclonal antibody, Aff - Purified

Product Images

XAGE1 antibody staining of paraffin embedded Human Testis at 3.8 ug/ml. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Specifications

Product Data

• Applications: ELISA, IHC
• Recommended Dilution:

  Peptide ELISA: 1/8000 (Detection limit).
  Western blot: Not yet tested - our routinely used western blotting protocol does not allow detection of proteins as small as the predicted size of approx. 8.0kDa according to NP_597673. Therefore we cannot recommend an optimal concentration and the product is investigative grade. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?
  Immunohistochemistry on Paraffin Sections: 3-6 μg/ml. paraffin embedded Human testis shows polar cytoplasm staining in primary spermatocytes.

• Reactivities: Human
• Host: Goat
• Clonality: Polyclonal
• Immunogen: Synthetic peptide with sequence from the C-Terminus of Human XAGE1D / GAGED2 (NP_597673.1).
• Specificity:
  This antibody is expected to recognise Isoform d (NP_597673.1) only.
  Reported variants represent identical protein (NP_597673.1; NP_001091067.1; NP_001091074.1; NP_001091062.1; NP_001091065.1).
• Formulation: Tris saline, pH~7.3
  State: Aff - Purified
  State: Liquid purified Ig fraction
  Stabilizer: 0.5% BSA
  Preservative: 0.02% Sodium Azide
• Concentration: lot specific
• Purification: Immunoaffinity Chromatography
• Conjugation: Unconjugated
• Storage:

  Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.

• Stability: Shelf life: one year from despatch.

• Gene Name: X antigen family member 1E
• Database Link:
  Entrez Gene 653067 Human
  UniProt ID Q9HD64
• Background: XAGE1 is a member of the XAGE subfamily, which belongs to the GAGE family. The GAGE genes are expressed in a variety of tumors and in some fetal and reproductive tissues. XAGE1 is strongly expressed in Ewing's sarcoma, alveolar rhabdomyosarcoma and normal testis. XAGE1 contains a nuclear localization signal and shares a sequence similarity with other GAGE/PAGE proteins. Because of the expression pattern and the sequence similarity, this protein also belongs to a family of CT (cancer-testis) antigens. Alternative splicing of XAGE1 generates 3 transcript variants, and one of which includes 2 transcripts generated from alternate transcription initiation sites.
• Synonyms: GAGED2, XAGE1, XAGE1A, XAGE1B, XAGE1C, XAGE1D, XAGE1E, CT12.1