P2RX4 Goat Polyclonal Antibody

CAT#: AP31932PU-N

P2RX4 goat polyclonal antibody, Aff - Purified


USD 465.00

5 Days*

Size
    • 100 ug

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Specifications

Product Data
Applications ELISA, IF, WB
Recommended Dilution

Peptide ELISA: 1/16000 (Detection limit). 
Western Blot: Approx 48-50kDa band observed in lysates of cell line A549 (calculated MW of 43.4kDa according to Human NP_002551.2). The observed molecular weight corresponds to earlier findings with different antibodies from other commercial sources. Recommended concentration: 0.3-1µg/ml. Primary incubation 1 hour at room temperature. Preliminary testing was unsuccessful on Mouse and Rat Kidney and Liver for this particular batch. 
Immunofluorescence: 10 µg/ml. Strong expression of the protein seen in the cytoplasm of A431 cells.

Immunohistochemistry on Paraffin Sections: 5 µg/ml in  Paraffin embedded Human Uterus.

Reactivities Bovine, Canine, Human, Mouse, Porcine, Rat
Host Goat
Clonality Polyclonal
Immunogen Peptide with sequence from the internal region of the protein sequence according to NP_035156.2.
Specificity This antibody recognizes P2rx4
Formulation Tris saline, pH~7.3
State: Aff - Purified
State: Liquid purified Ig fraction
Stabilizer: 0.5% BSA
Preservative: 0.02% Sodium Azide
Concentration lot specific
Purification Affinity Chromatography
Conjugation Unconjugated
Storage

Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.

Stability Shelf life: one year from despatch.

Predicted Protein Size 43.5 kDa (NP_002551.2)
Background The product of this gene belongs to the family of purinoceptors for ATP. This receptor functions as a ligand-gated ion channel with high calcium permeability. The main pharmacological distinction between the members of the purinoceptor family is the relative sensitivity to the antagonists suramin and PPADS. The product of this gene has the lowest sensitivity for these antagonists. Multiple alternatively spliced transcript variants have been identified for this gene although their full-length natures have not been determined.
Synonyms P2X purinoceptor 4, ATP receptor, Purinergic receptor
Reference Data

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.