ALB Rabbit Polyclonal Antibody

CAT#: AP31552SU-N

ALB rabbit polyclonal antibody, Serum

Specifications

Product Data

• Applications: ID, IP, R
• Recommended Dilution: This antibody can be used as precipitating antiserum to identify or measure Canine (Dog) Albumin by a variety of Immunodiffusion techniques, including Immunoelectrophoresis, Single and Double Radial Immunodiffusion (Mancini, Ouchterlony) and Electroimmunodiffusion (Laurell). It has not been tested for use in nephelometry, ELISA or Immunochemistry, but this does not exclude such use if proper controls are included.
  Recommended Dilutions:
  Immunoelectrophoresis: Use 2 μl serum, plasma or equivalent against 120 μl antiserum.
  Double Radial Immunodiffusion (Ouchterlony): Use a rosette arrangement with 10 μl antiserum in a 3 mm diameter centre well and 2 μl serum samples (neat and serially diluted) in 2 mm diameter peripheral wells.
  Single Radial Immunodiffusion/Electroimmunodiffusion: Use 0.5 to 1.0 percent antiserum in the agar gel.
• Reactivities: Canine
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Highly purified albumin isolated from Canine (Dog) serum. Freund’s complete adjuvant is used in the first step of the immunization procedure.
• Specificity: The defined antibody specificity is directed to Albumin as tested against Canine (Dog) sera. In immunoelectrophoresis and Double Radial Immunodiffusion (Ouchterlony), using various antiserum concentrations against appropriate concentrations of the immunogen, a single characteristic precipitin line is obtained which shows a reaction of identity with the precipitin lines obtained against Canine (Dog) serum and the purified Albumin.
  Cross-reactivity: Inter-species cross-reactivity is a normal feature of antibodies to serum proteins, since homologous proteins of different species frequently share antigenic determinants. The degree of cross-reactivity is also dependent on the concentrations of the reactants and the sensitivity of the assay arrangement. This antiserum has not been tested for cross-reactivity.
• Formulation: State: Serum
  State: Lyophilized (delipidated, heat inactivated) stable whole antiserum without preservatives
• Reconstitution Method: Restore by adding 1 ml of sterile distilled water
• Concentration: Total protein and IgG concentrations in the antiserum are comparable to those in pooled rabbit serum. No foreign proteins added.
• Conjugation: Unconjugated
• Storage: Store lyophilized at 2-8°C and reconstituted at 2-8°C for one week or (in aliquots) at -20°C for longer.
  Avoid Repeated thawing and freezing.
• Stability: Shelf life: one year from despatch.
• Database Link:
  Entrez Gene 403550 Dog
  UniProt ID P49822
• Background: Serum albumin is a class of simple, water-soluble proteins that can be coagulated by heat and are found in egg white, blood serum, milk, and many other animal and plant tissues.
  Serum albumin, often referred to simply as albumin, is the most abundant plasma protein in humans and other mammals. Albumin is essential for maintaining the osmotic pressure needed for proper distribution of body fluids between intravascular compartments and body tissues. It also has a role in transporting many small molecules in the blood (for example, bilirubin, calcium, progesterone, and drugs).
• Synonyms: ALB, BSA, HSA, Serum Albumin
• Note: Antibody titre: Precipitin titre not less than 1/28 when tested against normal Canine serum in agar block immunodiffusion titration.
  Adsorption: Immunoaffinity adsorbed using insolubilized antigens as required, to eliminate antibodies reacting with other serum proteins. The use of insolubilized adsorption antigens prevents the presence of excess adsorbent protein or immune complexes in the antiserum.