IDH1 Goat Polyclonal Antibody

CAT#: AP31099PU-N

IDH1 goat polyclonal antibody, Aff - Purified

Product Images

IDH1 antibody staining of S. cerevisiae lysate at 0.5 ug/ml (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Specifications

Product Data

• Applications: ELISA, WB
• Recommended Dilution: Peptide ELISA: Detection Limit: 1/16000.
  Western Blot: 0.3-1 µg/ml. A band of ~38kDa is observed in wildtype lysates of S. cerevisiae (Data kindly provided by F. Reggiiori, University Medical Centre Utrecht, Netherlands).
• Reactivities: Yeast
• Host: Goat
• Clonality: Polyclonal
• Immunogen: Peptide with sequence from the Internal region of the protein sequence according to NP_014361.1.
• Specificity: Recognizes IDH1 (Yeast).
• Formulation: Tris saline, pH~7.3
  State: Aff - Purified
  State: Liquid purified Ig fraction
  Stabilizer: 0.5% BSA
  Preservative: 0.02% Sodium Azide
• Concentration: lot specific
• Purification: Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Database Link:
  UniProt ID P28834
• Background: Isocitrate dehydrogenases catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. These enzymes belong to two distinct subclasses, one of which utilizes NAD(+) as the electron acceptor and the other NADP(+). Five isocitrate dehydrogenases have been reported: three NAD(+)-dependent isocitrate dehydrogenases, which localize to the mitochondrial matrix, and two NADP(+)-dependent isocitrate dehydrogenases, one of which is mitochondrial and the other predominantly cytosolic. Each NADP(+)-dependent isozyme is a homodimer. IDH1 is the NADP(+)-dependent isocitrate dehydrogenase found in the cytoplasm and peroxisomes.
  IDH1 contains the PTS-1 peroxisomal targeting signal sequence. The presence of this enzyme in peroxisomes suggests roles in the regeneration of NADPH for intraperoxisomal reductions, such as the conversion of 2, 4-dienoyl-CoAs to 3-enoyl-CoAs, as well as in peroxisomal reactions that consume 2-oxoglutarate, namely the alpha-hydroxylation of phytanic acid. The cytoplasmic enzyme serves a significant role in cytoplasmic NADPH production.
• Synonyms: Isocitric dehydrogenase, YNL037C
• Note: Calculated Molecular Weight: 39.3kDa (NP_014361.1).