Il5 Rabbit Polyclonal Antibody

CAT#: AP23789PU-S

Il5 rabbit polyclonal antibody, Aff - Purified

Specifications

Product Data

• Applications: ELISA, WB
• Recommended Dilution: Sandwich ELISA: To detect Mouse IL-5 by Sandwich ELISA (using 100μl/well antibody solution) a concentration of 0.5-2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Mouse IL5 (AP23789BT-N or AP23789BT-S) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant Mouse IL-5.
  Western Blot: To detect Mouse Il-5 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant Mouse IL-5 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
• Reactivities: Mouse
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Highly pure recombinant Murine IL-5
• Specificity: Recognizes Interleukin-5/IL5.
• Formulation: PBS, pH 7.2 without preservatives
  State: Aff - Purified
  State: Lyophilized (sterile filtered) purified Ig fraction
• Reconstitution Method: Centrifuge vial prior to opening. Restore in sterile water to a concentration of 0.1-1.0 mg/ml.
• Purification: Affinity Chromatography employing an immobilized Murine IL-5 matrix
• Conjugation: Unconjugated
• Storage: Store the lyophilized antibody at -20°C.
  Following reconstitution it is stable for two weeks at 2-8°C.
  Frozen aliquots are stable for 6 months when stored at -20°C.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: One year from despatch
• Gene Name: interleukin 5
• Database Link:
  Entrez Gene 16191 Mouse
  UniProt ID P04401
• Background: Interleukin 5 (IL5) is a cytokine produced primarily by activated T lymphocytes. It exists as an antiparallel disulfide linked homodimeric glycoprotein with 115 amino acid residues in each chain. Known also as EDF (eosinophil differentiating factor), functions predominantly as an eosinophilopoietic factor. Analysis of its crystal structure reveals a novel two domain structure, with each domain showing significant homology to the cytokine fold in GMCSF, MCSF, IL2, IL4 and growth hormone. Human and Mouse IL5 have 70% amino acid sequence homology.
• Synonyms: IL-5, B-cell differentiation factor I, Eosinophil differentiation factor, T-cell replacing factor, TRF