IDN3 (NIPBL) (889-902) Goat Polyclonal Antibody

CAT#: AP23654PU-N

IDN3 (NIPBL) (889-902) goat polyclonal antibody, Aff - Purified

Product Images

AP23654PU-N Negative Control showing staining of paraffin embedded Human Cerebellum, with no primary antibody.

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  AP23654PU-N (2µg/ml) staining of paraffin embedded Human Kidney. Microwaved antigen retrieval with citrate buffer pH 6, HRP-staining.

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  AP23654PU-N Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (4ug/ml), showing nuclear and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (4ug/ml).

Specifications

Product Data

• Applications: ELISA, IF, IHC
• Recommended Dilution: Peptide ELISA: Detection Limit: 1/32000.
  Immunohistochemistry on Paraffin Sections: 2 µg/ml.
  Immunofluorescence: Strong expression of the protein seen in the cytoplasm and nucleus of HeLa cells. Recommended concentration: 10µg/ml.
• Reactivities: Canine, Human, Bovine, Mouse, Rat
• Host: Goat
• Clonality: Polyclonal
• Immunogen: Peptide with sequence from the internal region of the protein sequence according to NP_597677.2 and NP_056199.2.
• Specificity: This antibody is expected to recognize both reported isoforms (NP_597677.2 and NP_056199.2).
• Formulation: Tris saline, pH 7.3 containing 0.02% Sodium Azide as preservative and 0.5% BSA as stabilizer.
  State: Aff - Purified
  State: Liquid purified IgG fraction
• Concentration: lot specific
• Purification: Ammonium Sulphate Precipitation followed by antigen Affinity Chromatography using the immunizing peptide.
• Conjugation: Unconjugated
• Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: NIPBL, cohesin loading factor
• Database Link:
  Entrez Gene 25836 Human
  UniProt ID Q6KC79
• Synonyms: IDN3,Delangin, SCC2 homolo