TDH1 Rabbit Polyclonal Antibody

CAT#: AP21309PU-N

TDH1 rabbit polyclonal antibody, Aff - Purified

Specifications

Product Data

• Applications: ELISA, ID, IF, IP, WB
• Recommended Dilution: This product is intended for use in precipitating and non-precipitating antibody-binding assays (such as e.g., ELISA and Western blotting and Immunofluorescence or Histochemical techniques), to prepare an insoluble immuno-affinity adsorbent, for labelling with a marker of
  the customer’s own choice.
  Working dilutions in non-precipitating antibody-binding techniques: 1/1,000-1/20,000.
• Reactivities: Bakers Yeast
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Glyceraldehyde-3-Phosphate Dehydrogenase isolated and purified from Baker's Yeast.
  Freund’s complete adjuvant is used in the first step of the immunization procedure.
• Specificity: The antibody recognizes GAPDH from Baker's Yeast.
  The reagents were evaluated for potency, purity and specificity using most or all of the following techniques: Immunoelectrophoresis, Cross-Immunoelectrophoresis, single Radial Immunodiffusion (Ouchterlony), block titration, ELISA, Immunoblotting and Enzyme Inhibition.
  Cross-reactivities against enzymes of other sources may occur but have not been determined.
• Formulation: PBS, pH 7.2 stabilized with Dextran without preservatives and foreign protein
  State: Aff - Purified
  State: Lyophilized purified IgG fraction
• Reconstitution Method: Restore by adding 0.5 ml of sterile distilled water
• Concentration: lot specific
• Purification: Solid Phase Affinity Chromatography
• Conjugation: Unconjugated
• Storage: Store the antibody lyophilized at 2-8°C and reconstituted at 2-8°C for one week or (in aliquots) at -20°C for longer.
  If a slight precipitation occurs upon storage, this should be removed by centrifugation.
• Stability: Shelf life: one year from despatch.
• Database Link:
  UniProt ID P00360
• Background: Glyceraldehyde 3 phosphate dehydrogenase (GAPDH) is well known as one of the key enzymes involved in glycolysis. Besides its functioning as a glycolytic enzyme in cytoplasm, recent evidence suggest that mammalian GAPDH is also involved in a great number of intracellular proceses such as membrane fusion, microtubule bundling, phosphotransferase activity, nuclear RNA export, DNA replication, and DNA repair. During the last decade a lot of findings appeared concerning the role of GAPDH in different pathologies including prostate cancer progression, programmed neuronal cell death, age-related neuronal diseases, such as Alzheimer's and Huntington's disease. GAPDH is constitutively expressed in almost all tissues at high levels, therefore becoming the marker of choice when a loading control in Western blotting is required. Some physiological factors, such as hypoxia and diabetes, increase GAPDH expression in certain cell types.
• Synonyms: GAPD, CDABP0047