lacZ Rabbit Polyclonal Antibody

CAT#: AP21258BT-N

lacZ rabbit polyclonal antibody, Biotin

Specifications

Product Data

• Applications: ELISA, ID, IF, IP, R, WB
• Recommended Dilution: This product is intended for use in precipitating and non-precipitating antibody-binding assays (such as e.g., ELISA and Western blotting and Immunofluorescence or Histochemical techniques).
  Working Dilutions:
  Non-precipitating antibody-binding techniques: 1/10,000-1/50,000.
• Reactivities: Escherichia coli
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Beta-Galactosidase is isolated and purified from Escherichia coli.
  Freund’s complete adjuvant is used in the first step of the immunization procedure.
• Specificity: This antibody recognizes Beta-Galactosidase from Escherichia coli.
  The reagents were evaluated for potency, purity and specificity using most or all of the following techniques: Immunoelectrophoresis, Cross-Immunoelectrophoresis, Single Radial Immunodiffusion (Ouchterlony), block titration, ELISA, Immunoblotting and enzyme inhibition.
  Cross-reactivities against enzymes of other sources may occur but have not been determined.
• Formulation: PBS, pH 7.2 without preservatives and foreign proteins
  Label: Biotin
  State: Lyophilized hyperimmune IgG fraction
  Molar radio: Biotin/IgG ~3.1
• Concentration: lot specific
• Purification: Ammonium Sulphate Precipitation and Ion Exchange Chromatography
• Conjugation: Biotin
• Storage: Store the antibody lyophilized at 2-8°C and reconstituted at 2-8°C for one week or (in aliquots) at -20°C for longer.
  If a slight precipitation occurs upon storage, this should be removed by centrifugation.
• Stability: Shelf life: one year from despatch.
• Database Link:
  UniProt ID P00722
• Synonyms: Beta-Gal tag, lacZ tag, b0344, JW0335, Beta-Gal Fusion Protein, Lactase