Tryptophan Hydroxylase (TPH1) Rabbit Polyclonal Antibody

CAT#: AP21097PU-N

Tryptophan Hydroxylase (TPH1) rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot (WB) analysis of TPH1 antibody (Cat.-No.: AP21097PU-N) in extracts from Jurkat cells.

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  Immunohistochemistry (IHC) analyzes of TPH1 antibody (Cat.-No.: AP21097PU-N) in paraffin-embedded human lung carcinoma tissue.

Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution: Western blot: 1/500 - 1/1000.
  Immunohistochemistry on paraffin sections: 1/50 - 1/200.
  Immunofluorescence: 1/50 - 1/200.
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Clonality: Polyclonal
• Specificity: This antibody detects endogenous levels of TPH1 protein.
  (region surrounding Val256)
• Formulation: Phosphate buffered saline (PBS), pH 7.2.
  State: Aff - Purified
  State: Liquid purified Ig fraction
  Preservative: 0.05% sodium azide
• Concentration: 1.0 mg/ml
• Purification: Affinity chromatography (> 95% (by SDS-PAGE)
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: ~ 51 kDa
• Gene Name: tryptophan hydroxylase 1
• Database Link:
  Entrez Gene 21990 MouseEntrez Gene 24848 RatEntrez Gene 7166 Human
  UniProt ID P17752
• Background: Phenylalanine hydroxylase (PAH), tyrosine hydroxylase (TH) and tryptophan hydroxylase (TPH) comprise a small family of monooxygenases that use tetrahydropterine as a cofactor during the catabolism of aromatic L-amino acids. PAH, TH and TPH all contain catalytic domains with an aminoterminal regulatory domain and a short carboxy-terminal tetramerization domain. Each of these enzymes also contains a single ferrous iron atom, which is bound to two histidines and a glutamate, and is likely to be involved in the formation of the hydroxylating intermediate. TPH is both the first- and rate-limiting-step in the biosynthesis of serotonin in the central nervous system and melatonin in the pineal gland. Alteration of TPH function may be a key factor in the pathology of several neuropsychiatric disorders associated with serotonin, including depression, aggression, alcoholism and schizophrenia. For instance, L-DOPA, which is used as a common therapy for Parkinson’s disease (PD) patients, inhibits TPH function which, subsequently, is thought to contribute to the onset of depression in PD patients.
• Synonyms: Tryptophan 5-monooxygenase 1, TPRH, TRPH

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Metabolic pathways, Tryptophan metabolism