KSR2 Rabbit Polyclonal Antibody

CAT#: AP20447PU-N

KSR2 rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot analysis of KSR2 in extracts from 293 cells using Kinase suppressor of Ras 2 antibody.

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  Immunohistochemistry analysis of KSR2 in human brain tissue (Formalin-fixed, Paraffin-embedded) using Kinase suppressor of Ras 2 antibody.

Specifications

Product Data

• Applications: ELISA, IF, IHC, WB
• Recommended Dilution: Western Blot: 1/500-1/1000.
  Immunofluorescence: 1/50-1/200.
  Immunohistochemistry on Paraffin Sections: 1/50-1/200.
• Reactivities: Human, Mouse
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Synthetic peptide, corresponding to amino acids 662-724 of Human Ksr2.
• Specificity: This antibody detects endogenous levels of Kinase suppressor of Ras 2.
  (region surrounding Ile694)
• Formulation: PBS, pH 7.2
  State: Aff - Purified
  State: Liquid purified Ig fraction (> 95% by SDS-PAGE)
  Preservative: 0.05% Sodium Azide
• Concentration: 1.0 mg/ml
• Purification: Immunoaffinity Chromatography
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: ~ 108 kDa
• Database Link:
  Entrez Gene 283455 Human
  UniProt ID Q6VAB6
• Background: Kinase suppressor of Ras (KSR) and MAP kinase kinase kinase (MEKK3) are integral members of the MAP kinase pathway. Ksr is a conserved protein that positively regulates Ras signaling and may function as a scaffold for Raf, MEK and ERK. There are two types of Ksr proteins: Ksr-1 and Ksr-2. These two are individually necessary for a few specific Ras-dependent processes, but they are required together for most aspects of Ras-mediated signaling. Ksr-2 plays a key role in Ras-mediated signaling during germline meiotic progression and functions redundantly with Ksr-1 during the development of the excretory system pathway, hermaphrodite vulva and male spicules. Ksr-2 also functions as a negative regulator of the MEKK3-mediated activation of the MAP kinase pathways (specifically ERK and JNK) and of the NFκB pathways, and it simultaneously inhibits MEKK3-mediated IL-8 production.
• Synonyms: KSR2, KSR-2, hKSR2, hKSR-2