CEBP Alpha (CEBPA) Rabbit Polyclonal Antibody

CAT#: AP20354PU-N

CEBP Alpha (CEBPA) rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot (WB) analysis of C/EBP-a antibody (Cat.-No.: AP20354PU-N) at 1/500 dilution Lane 1:HEK293T whole cell lysate Lane 2:Raw264.7 whole cell lysate Lane 3:PC12 whole cell lysate

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  Immunohistochemistry analyzes of C/EBP-a antibody (Cat.-No.: AP20354PU-N) in paraffin-embedded human colon carcinoma tissue.

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: Western blot: 1/500-1/1000.
  Immunohistochemistry on paraffin sections: 1/50-1/200.
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Clonality: Polyclonal
• Specificity: This antibody detects endogenous levels of C/EBP-α protein.
  (region surrounding Pro218)
• Formulation: Phosphate buffered saline (PBS), pH 7.2
  State: Aff - Purified
  State: Liquid purified Ig fraction
  Preservative: 0.05% sodium azide
• Concentration: 1.0 mg/ml
• Purification: Affinity-chromatography using epitope-specific immunogen; purity is > 95% (by SDS-PAGE)
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: ~ 30, 43 kDa
• Gene Name: CCAAT/enhancer binding protein alpha
• Database Link:
  Entrez Gene 12606 MouseEntrez Gene 24252 RatEntrez Gene 1050 Human
  UniProt ID P49715
• Background: C/EBP α regulates gene expression in a variety of tissues including liver, adipose, lung and intestine. C/EBP α is a basic region/leucine zipper transcription factor selectively expressed during the differentiation of liver, adipose tissue, blood cells and the endocrine pancreas. C/EBP α uses a bipartite structural motif to bind DNA and appears to function exclusively in terminally differentiated, growtharrested cells. In the liver, C/EBP α is a transactivator of several genes, which are regulated by growth hormone. Growth hormone enhances not only the levels of C/EBP α mRNA and protein, but also the DNA-binding activity of C/EBP α. C/EBP α functions as an important transcription factor that regulates different genes, including Prolactin gene expression.
• Synonyms: C/EBP alpha

Reference Data

• Protein Families: Druggable Genome, ES Cell Differentiation/IPS
• Protein Pathways: Acute myeloid leukemia, Pathways in cancer