P4HB (C-term) Rabbit Polyclonal Antibody

CAT#: AP17615PU-N

P4HB (C-term) rabbit polyclonal antibody

Product Images

Western blot analysis of P4HB antibody (C-term) in CEM, HepG2, A2058 cell line lysates (35 ug/lane). P4HB (arrow) was detected using the purified Pab. (2ug/ml)

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  P4HB antibody staining of Formalin-Fixed and paraffin-Embedded Human hepatocarcinoma using peroxidase-conjugated to the secondary antibody, followed by DAB staining. Clinical relevance has not been evaluated.

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  Flow Cytometric analysis of K562 cells using P4HB Antibody (C-term) (bottom histogram) compared to a Negative Control cell (top histogram). FITC-conjugated goat-anti-Rabbit secondary antibodies were used for the analysis.

Specifications

Product Data

• Applications: FC, IHC, WB
• Recommended Dilution: ELISA: >1/1,000 (peptide ELISA only)
  Western blotting: 1/500-1/1000.
  Flow Cytometry: 1/10-1/50.
  Immunohistochemistry: 1/50-1/100.
• Reactivities: Human
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: KLH conjugated synthetic peptide between 436~464 amino acids from the C-terminal region of Human P4HB.
• Specificity: This antibody recognizes Protein Disulfide Isomerase.
• Formulation: PBS with 0.09% (W/V) Sodium Azide as preservative
  State: Liquid purified Ig fraction
• Concentration: lot specific
• Purification: Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS
• Conjugation: Unconjugated
• Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: prolyl 4-hydroxylase subunit beta
• Database Link:
  Entrez Gene 5034 Human
  UniProt ID P07237
• Background: P4HB is a highly abundant multifunctional enzyme that belongs to the protein disulfide isomerase family. When present as a tetramer consisting of two alpha and two beta subunits, this enzyme is involved in hydroxylation of prolyl residues in preprocollagen. This enzyme is also a disulfide isomerase containing two thioredoxin domains that catalyze the formation, breakage and rearrangement of disulfide bonds. Other known functions include its ability to act as a chaperone that inhibits aggregation of misfolded proteins in a concentration-dependent manner, its ability to bind thyroid hormone, its role in both the influx and efflux of S-nitrosothiol-bound nitric oxide, and its function as a subunit of the microsomal triglyceride transfer protein complex.
• Synonyms: ERBA2L, PDIA1, PO4DB, Protein disulfide-isomerase, ER marker

Reference Data

• Protein Families: Druggable Genome