Pumilio 2 (PUM2) Rabbit Polyclonal Antibody

CAT#: AP14501PU-N

Pumilio 2 (PUM2) rabbit polyclonal antibody, Aff - Purified

Product Images

Figure 1. Western blot analysis of PUM2 (arrow) using PUM2 Antibody. 293 cell lysates (2 ug/lane) either non-transfected (Lane 1) or transiently transfected with the PUM2 gene (Lane 2) (Origene Technologies).

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  Figure 2. Formalin-fixed and paraffin-embedded human prostate carcinoma reacted with PUM2 Antibody (S182), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

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  Figure 3. Flow cytometric analysis of HepG2 cells using PUM2 Antibody (S182) (Bottom Histogram) compared to a Negative Control cell (Top Histogram). FITC-conjugated Goat-anti-Rabbit secondary antibodies were used for the analysis.

Specifications

Product Data

• Applications: FC, IHC, WB
• Recommended Dilution: ELISA: 1/1,000.
  Western blot: 1/50~1/100.
  Immunohistochemistry: 1/10~1/50.
  Flow Cytometry: 1/10~1/50.
• Reactivities: Human
• Host: Rabbit
• Isotype: Ig
• Clonality: Polyclonal
• Immunogen: KLH conjugated synthetic peptide between 159-189 amino acids from Human PUM2
• Specificity: This antibody recognizes Pumilio 2 (PUM2).
• Formulation: PBS with 0.09% (W/V) Sodium Azide as preservative.
  State: Aff - Purified
  State: Liquid purified Ig fraction.
• Concentration: lot specific
• Purification: Protein A Chromatography followed by peptide affinity purification.
• Conjugation: Unconjugated
• Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: pumilio RNA binding family member 2
• Database Link:
  Entrez Gene 23369 Human
  UniProt ID Q8TB72
• Background: PUM2 is a sequence-specific RNA-binding protein that regulates translation and mRNA stability by binding the 3' UTR of mRNA targets. Its interactions and tissue specificity suggest that it may be required to support proliferation and self-renewal of stem cells by regulating the translation of key transcripts.
• Synonyms: Pumilio homolog 2, PUM-2, Pumilio-2, PUMH2, KIAA0235
• Note: Calculated Molecular Weight: 114216 Da