Zinc finger MIZ domain containing protein 1 (ZMIZ1) (C-term) Rabbit Polyclonal Antibody

CAT#: AP13210PU-N

Zinc finger MIZ domain containing protein 1 (ZMIZ1) (C-term) rabbit polyclonal antibody, Purified

Product Images

Western blot using ZMIZ1 / RAI17 Antibody (C-term) to detect Rai17 in Placenta lysate.

• 

  Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining.

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution:

  ELISA: 1/1,000.
  Western Blot: 1/50-1/100.
  Immunohistochemistry on Paraffin Sections: 1/50-1/100.

• Reactivities: Human
• Host: Rabbit
• Isotype: Ig
• Clonality: Polyclonal
• Immunogen: KLH conjugated synthetic peptide between 596~626 amino acids from the C-terminal region of Human RAI17
• Specificity: This antibody reacts to RAI17.
• Formulation: PBS with 0.09% (W/V) Sodium Azide as preservative
  State: Purified
  State: Liquid purified Ig fraction
• Concentration: lot specific
• Purification: Protein G Chromatography, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS
• Conjugation: Unconjugated
• Storage:

  Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.

• Stability: Shelf life: one year from despatch.

• Gene Name: zinc finger MIZ-type containing 1
• Database Link:
  Entrez Gene 57178 Human
  UniProt ID Q9ULJ6
• Background: Retinoic acid plays a critical role in development, cellular growth, and differentiation and induces the expression of a variety of genes. RAI17 expression is induced by retinoic acid and is predominantly expressed in heart, brain and ovaries. Within brain, highest expression is in amygdala. The deduced 1,067-amino acid protein contains an MSX-interacting zinc finger (MIZ) domain, a nuclear localization signal sequence, and 2 proline-rich regions. A strong intrinsic transactivation domain is identified in the C-terminal proline-rich region. RAI17 expression is detected in various cancer cell lines. RAI17 colocalizes with endogenous androgen receptor (AR) in the nuclei of prostate epithelial cells from human tissue samples. In human prostate cancer cells, RAI17 increases the transcriptional activity of AR. Studies using sumoylation-deficient AR mutants suggest that the increase of AR activity by RAI17 is dependent upon receptor sumoylation.
• Synonyms: KIAA1224, ZIMP10