PIK3CD pTyr524 Rabbit Polyclonal Antibody

CAT#: AP12879PU-N

PIK3CD pTyr524 rabbit polyclonal antibody, Aff - Purified

Product Images

Dot blot analysis of anti-PIK3CD-pTyr524 Phospho-specific Pab (Cat#AP12879PU-N) on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.

Specifications

Product Data

• Recommended Dilution: ELISA: 1/1,000.
  Dot Blot: 1/500.
• Reactivities: Human
• Host: Rabbit
• Isotype: Ig
• Clonality: Polyclonal
• Immunogen: This antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding Y524 of human PIK3CD.
• Specificity: This antibody detects PIK3CD pTyr524.
  Predicted to cross react with Mouse (100% Antigen Homology).
• Formulation: PBS with 0.09% (W/V) Sodium Azide as preservative.
  State: Aff - Purified
  State: Liquid purified Ig fraction.
• Concentration: lot specific
• Purification: Affinity purification in a 2-step procedure with the control and phosphorylated peptides. The phospho-specific antibody is eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
• Conjugation: Unconjugated
• Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit delta
• Database Link:
  Entrez Gene 5293 Human
  UniProt ID O00329
• Background: Phosphoinositide 3-kinases (PI3Ks) phosphorylate the 3-prime OH position of the inositol ring of inositol lipids. See MIM 602838. The class I PI3Ks display a broad phosphoinositide lipid substrate specificity and include p110-alpha , p110-beta, and p110-gamma. p110-alpha and p110-beta interact with SH2/SH3-domain-containing p85 adaptor proteins and with GTP-bound Ras.
• Synonyms: PI3-kinase subunit delta, PI3K-delta, PtdIns-3-kinase subunit delta
• Note: Molecular weight: 119479 Da