LECT1 (CNMD) (C-term) Rabbit Polyclonal Antibody

CAT#: AP12354PU-N

LECT1 (CNMD) (C-term) rabbit polyclonal antibody, Purified

Product Images

Western blot analysis of anti-LECT1 Antibody (C-term) in NCI-H460 cell line lysates (35ug/lane).LECT1 (arrow) was detected using the purified Pab.

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  Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with LECT1 antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: Peptide ELISA: 1/1,000.
  Western Blot: 1/50-1/100.
  Immunohistochemistry: 1/10-1/50.
• Reactivities: Human
• Host: Rabbit
• Isotype: Ig
• Clonality: Polyclonal
• Immunogen: KLH conjugated synthetic peptide between 253-281 amino acids from the C-terminal region of human LECT1.
• Specificity: This antibody detects LECT1 (C-term).
  Predicted to Cross React with Mouse Rabbit (100% Antigen Homology).
• Formulation: PBS with 0.09% (W/V) Sodium Azide as preservative.
  State: Purified
  State: Liquid purified Ig fraction.
• Concentration: lot specific
• Purification: Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Conjugation: Unconjugated
• Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: leukocyte cell derived chemotaxin 1
• Database Link:
  Entrez Gene 11061 Human
  UniProt ID O75829
• Background: LECT1 a glycosylated transmembrane protein that is cleaved to form a mature, secreted protein. The N-terminus of the precursor protein shares characteristics with other surfactant proteins and is sometimes called chondrosurfactant protein although no biological activity has yet been defined for it. The C-terminus of the precursor protein contains a 25 kDa mature protein called leukocyte cell-derived chemotaxin-1 or chondromodulin-1. The mature protein promotes chondrocyte growth and inhibits angiogenesis. This protein is expressed in the avascular zone of prehypertrophic cartilage and its expression decreases during chondrocyte hypertrophy and vascular invasion. The mature protein likely plays a role in endochondral bone development by permitting cartilaginous anlagen to be vascularized and replaced by bone. It may be involved also in the broad control of tissue vascularization during development.
• Synonyms: LECT1, CHMI, Chondromodulin1, Chondromodulin-I, ChM-I, Leukocyte cell-derived chemotaxin 1
• Note: Molecular weight: 37102 Da