AMFR (N-term) Rabbit Polyclonal Antibody

CAT#: AP12067PU-N

AMFR (N-term) rabbit polyclonal antibody, Purified

Product Images

(LEFT) Western blot analysis of anti-AMFR Antibody (N-term) in CEM cell line lysate (35ug/lane). AMFR (arrow) was detected using the purified Pab. (RIGHT) Western blot analysis of AMFR (arrow) using AMFR Antibody (N-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the AMFR gene (Lane 2) (Origene Technologies).

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  (LEFT) Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. (RIGHT) Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with AMFR Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: ELISA: 1/1,000.
  Western Blot: 1/100-1/500.
  Immunohistochemistry: 1/50-1/100.
• Reactivities: Human
• Host: Rabbit
• Isotype: Ig
• Clonality: Polyclonal
• Immunogen: This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the N-terminal region of human AMFR.
• Specificity: This antibody is specific to AMFR (N-term).
• Formulation: PBS with 0.09% (W/V) Sodium Azide as preservative.
  State: Purified
  State: Liquid purified Ig fraction.
• Concentration: lot specific
• Purification: Protein G Chromatography, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
• Conjugation: Unconjugated
• Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: Homo sapiens autocrine motility factor receptor, E3 ubiquitin protein ligase (AMFR)
• Database Link:
  Entrez Gene 267 Human
  UniProt ID Q9UKV5
• Background: Autocrine motility factor (AMF) is a protein secreted by tumor cells that stimulates tumor motility. The gene for AMFR encodes a 323-amino acid polypeptide that has a single transmembrane domain and several putative glycosylation sites. The protein sequence has some homology to human tumor protein p53.
• Synonyms: AMF receptor isoform 2, gp78, RING finger protein 45
• Note: Predicted Molecular weight: 34325 Da

Reference Data

• Protein Families: Druggable Genome, Transmembrane