SENP2 (C-term) Rabbit Polyclonal Antibody

CAT#: AP11235PU-N

SENP2 (C-term) rabbit polyclonal antibody, Purified

Product Images

(LEFT) The SENP2 C-term polyclonal antibody is used in Western blot to detect SENP2 in mouse heart tissue lysate. (RIGHT) Western blot analysis of anti-SENP2 Antibody (C-term) in HL60 cell line lysates (35ug/lane). SENP2 (arrow) was detected using the purified Pab.

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  Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: ELISA: 1/1,000.
  Western blot: 1/100-1/500.
  Immunohistochemistry: 1/50-1/100.
• Reactivities: Human, Mouse
• Host: Rabbit
• Isotype: Ig
• Clonality: Polyclonal
• Immunogen: This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the C-terminal region of human SENP2.
• Specificity: This antibody is specific to SENP2 (C-term).
• Formulation: PBS containing 0.09% (W/V) Sodium Azide as preservative.
  State: Purified
  State: Liquid purified Ig fraction.
• Concentration: lot specific
• Purification: Protein G Chromatography, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
• Conjugation: Unconjugated
• Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: SUMO1/sentrin/SMT3 specific peptidase 2
• Database Link:
  Entrez Gene 59343 Human
  UniProt ID Q9HC62
• Background: SUMO is a small ubiquitin-like protein that can be covalently conjugated to other proteins. SENP2 is one of a group of enzymes that process newly synthesized SUMO1, SUMO2, and SUMO3 into the conjugatable mature forms and catalyze the deconjugation of these same SUMO proteins from their targeted substrates. SENP2 may also down-regulate CTNNB1 levels and thereby modulate the Wnt pathway.
• Synonyms: Sentrin-specific protease 2, Smt3ip2, Axam2, KIAA1331
• Note: Predicted MW: 67881 Da