CFTR Rabbit Polyclonal Antibody

CAT#: AP10443SU-N

CFTR rabbit polyclonal antibody, Serum

Product Images

Western Blot using CFTR antibody Cat.-No AP10443SU-N at 1/500 dilution. Lane A: BHK (baby-Hamster kidney) cells. Lane B: BHK cells transfected with CFTR-3HA tag. Lane C: Immunoprecipitated with CFTR from BHK cells extract.

• 

  BHK cells expressing wild-type (wt) CFTR or mutated (rÎ?F508), were fixed, permeabilized, and CFTR protein was detected with the specific CFTR antibody Cat.-No AP10443SU-N

Specifications

Product Data

• Applications: ELISA, IF, IP, WB
• Recommended Dilution: ELISA: 1/2000-1/32000.
  Western Blot: 1/500-1/1000.
  Immunofluorescence.
  Immunoprecipitation.
• Reactivities: Human
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Synthetic peptides derived from C-terminal domain of Human CFTR protein
• Specificity: This antibody reacts with human 168 kDa CFTR.
• Formulation: Tris 0,1M, glycine 0,1M, sucrose 2%
  State: Serum
  State: Lyophilized serum
  Preservative: None
• Reconstitution Method: Restore in distilled water.
• Conjugation: Unconjugated
• Storage:

  Prior to reconstitution store the antibody at -20°C.
  Store reconstituted antibody at 2-8°C for one month or (in aliquots) at -20°C for longer
  Avoid repeated freezing and thawing.

• Stability: Shelf life: one year from despatch.

• Gene Name: cystic fibrosis transmembrane conductance regulator
• Database Link:
  Entrez Gene 1080 Human
  UniProt ID P13569
• Background: CFTR (cystic fibrosis transmembrane conductance regulator) is composed of two membrane-spanning domains (MSD), two nucleotide-binding domains (NBD), and an R domain. It is structurally similar to multidrug resistance (Mdr1) protein and both are members of the superfamily of ATP-binding cassette (ABC) transporters, also known as traffic ATPases, which are implicated in the movement of various substrates. The CFTR protein is a small conductance adenosine 3',5'-cyclic monophosphate (cAMP)-activated chloride ion channel found in the apical membranes of epithelia within the pancreas, airway, intestine, bile duct, sweat gland, and male genital ducts. CFTR is a valuable marker of human pancreatic duct cell development and differentiation.
• Synonyms: Cystic fibrosis transmembrane conductance regulator