Neurofilament (NEFM) Goat Polyclonal Antibody

CAT#: AP09628PU-N

Neurofilament (NEFM) goat polyclonal antibody, Aff - Purified

Product Images

Neurofilament M Antibody (0.03ug/ml) staining of Mouse Brain lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Specifications

Product Data

• Applications: ELISA, WB
• Recommended Dilution: Peptide ELISA: 1/128000.
  Western blot: 0.03-0.1 µg/ml. Approx 170-200kDa band observed in Mouse Brain lysates (calculated MW of 102kDa according to NP_005373.2). The observed molecular weight corresponds to earlier findings with different antibodies from other commercial sources.
• Reactivities: Bovine, Canine, Human, Mouse, Porcine, Rat
• Host: Goat
• Clonality: Polyclonal
• Immunogen: Peptide with sequence C-QASHITVERKDYLK, from the internal region of the protein sequence
• Specificity: This antibody is expected to recognize reported isoform 1 (NP_005373.2) only.
• Formulation: Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin
  State: Aff - Purified
  State: Liquid purified Ig fraction
• Concentration: lot specific
• Purification: Affinity chromatography
• Conjugation: Unconjugated
• Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: neurofilament, medium polypeptide
• Database Link:
  Entrez Gene 4741 Human
  UniProt ID P07197
• Background: Neurofilaments are the 10nm or intermediate filament proteins found specifically in neurons, and are composed predominantly of three major proteins called neurofilament light (NF-L), neurofilament medium (NF-M) and neurofilament heavy (NF-H). Neurofilament medium runs on SDS-PAGE gels in the range 145-170 kDa, with some variation in different species. Antibodies to this protein are useful to identify neurons and their processes in tissue sections and in tissue culture. Neurofilament medium can also be useful in studies of neurofilament accumulations seen in many neurological diseases, such as Lou Gehrig's disease or Alzheimer's disease.
• Synonyms: Neurofilament medium polypeptide, NF-M, NEF3, NEFM, Neurofilament 3, (Neuronal Marker)