RAB11FIP3 Rabbit Polyclonal Antibody

CAT#: AP09361PU-N

RAB11FIP3 rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot using affinity purified anti-FIP3 to detect FIP3 (arrowhead) in 3T3 cells (lane 1), HeLa cells (lane 2) and 293T cells (lane 3). The membrane was probed with the primary antibody at a 1:2,000 dilution.

Specifications

Product Data

• Applications: ELISA, WB
• Recommended Dilution: ELISA: 1/2,000 - 1/10,000.
  Western Blot: 1/200 - 1/2,000.
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Recombinant protein corresponding to amino acids spanning the carboxyl terminal one-third of human FIP3
• Specificity: This antibody is directed against FIP3 protein.
• Formulation: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
  State: Aff - Purified
  State: Liquid (sterile filtered) purified Ig fraction
  Preservative: 0.01% (w/v) Sodium Azide
• Concentration: lot specific
• Purification: Affinity chromatography
• Conjugation: Unconjugated
• Storage: Store the antibody at -20°C.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: RAB11 family interacting protein 3
• Database Link:
  Entrez Gene 9727 Human
  UniProt ID O75154
• Background: Rab11 Family-Interacting Protein 3 (FIP3)/Arfophilin-1 and FIP4/Arfophilin-2 are dual effectors for Rab11 and the ADP-ribosylation factor Arf6. Rab11 and Arf6 are involved in membrane delivery from recycling endosomes to the plasma membrane during cytokinesis. The dual Rab11/Arf6 binding proteins, FIP3 and FIP4, function in the delivery of recycling endosomes to the cleavage furrow and are, together with Rab11, essential for completion of abscission, the terminal step of cytokinesis. An interaction between Rab11-FIP3 and ASAP1 has also been identified (personal communication, P. Randazzo, CCR-NCI, Bethesda, MD). ASAP1 is an Arf GTPase-activating protein that has been implicated in the invasive properties of uveal melanoma and mammary carcinoma.
• Synonyms: Rab11-FIP3, FIP3-Rab11, Arfophilin-1, ARFO1, Eferin, KIAA0665