Vimentin (VIM) Rabbit Polyclonal Antibody

CAT#: AP08114PU-N

Vimentin (VIM) rabbit polyclonal antibody, Purified

Specifications

Product Data

• Applications: IHC
• Recommended Dilution: Immunohistochemistry: 1/50-1/100 using polymer system or streptavidin-biotin system.
  Formalin-fixed paraffin-embedded (FFPE) tissue sections and cell smears do not require antigen retrieval.
  Positive Control: Sarcomas, melanomas, Hela cells.
• Reactivities: Human
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: 14 amino acid peptide from C-terminal of Human Vimentin.
• Specificity: This antibody reacts with Human Vimentin. Other species not tested.
  Cellular Localization: Cytoplasmic.
• Formulation: 10mM PO4, 200mM NaCl, pH 7.4
  State: Purified
  State: Liquid purified Total Ig fraction
  Stabilizer: 1% BSA
  Preservative: 0.05% Sodium Azide
• Concentration: lot specific
• Purification: Protein A Chromatography
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: One year from despatch.
• Gene Name: vimentin
• Database Link:
  Entrez Gene 7431 Human
  UniProt ID P08670
• Background: Vimentin is 57 kDa intermediate filament proteins in mesenchymal cells. It is attached to the nucleus, endoplasmic reticulum and mitochondria either laterally or terminally. Vimentin is important in the differential diagnosis of undifferentiated neoplasm.
• Synonyms: VIM