eNOS (NOS3) pSer1177 Rabbit Polyclonal Antibody

CAT#: AP08011PU-S

eNOS (NOS3) pSer1177 rabbit polyclonal antibody, Aff - Purified

Product Images

Figure 1. Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Phospho-Ser1177 eNOS antibody

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  Figure 2. Immunofluorescence staining of methanol-fixed HeLa cells using phospho-Ser1177 eNOS antibody (Red).

Specifications

Product Data

• Applications: IF, IHC
• Recommended Dilution: Immunofluorescence: 1/100-1/200.
  Immunohistochemistry on Paraffin Embedded Sections: 1/50-1/100.
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: The antiserum was produced against synthesized phosphopeptide derived from human eNOS around the phosphorylation site of Serine 1177 (T-Q-Sp-F-S).
• Specificity: Phospho-eNOS antibody (AP08011PU) detects endogenous levels of eNOS only when phosphorylated at serine 1177.
• Formulation: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02% Sodium Azide as preservative and 50% Glycerol as stabilizer.
  State: Aff - Purified
  State: Liquid purified Ig fraction.
• Concentration: lot specific
• Purification: Immunoaffinity Chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
• Conjugation: Unconjugated
• Storage: Store the antibody (in aliquots) at -20°C.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: One year from despatch.
• Database Link:
  Entrez Gene 4846 Human
  UniProt ID P29474
• Background: eNOS is a calcium/calmodulin dependent enzyme which undergoes several post translational modifications, including acylation with myristate and palmitate, and phosphorylation on numerous residues. As with the other members of the NOS family, eNOS derives the diffusible multifunctional second messenger NO from L arginine through a series of reactions in which L citrulline is an intermediate. eNOS plays an important role in controlling vascular tone, platelet aggregation, and cardiac myocyte function.
• Synonyms: Endothelial NOS, EC-NOS, NOS type III, Constitutive NOS, eNOS