XAF1 Rabbit Polyclonal Antibody

CAT#: AP07806PU-N

XAF1 rabbit polyclonal antibody, Aff - Purified

Product Images

Figure 1. Staining XAF1 in Prostate by Immunohistochemistry using Formalin-Fixed Paraffin-Embedded (FFPE) tissue.

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: Immunohistochemistry on Paraffin Sections: 5 µg/ml.
  Western Blot: 0.5 - 2 µg/ml.
• Reactivities: Human, Mouse
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Synthetic Peptide corresponding to amino acids at the C-terminus of human XAF-1.
• Specificity: This antibody recognises XIAP-Associated Factor 1.
• Formulation: Phosphate Buffered Saline PBS containing 0.02% Sodium Azide as preservative.
  State: Aff - Purified
  State: Liquid purified IgG fraction.
• Concentration: lot specific
• Purification: Immunoaffinity Chromatography.
• Conjugation: Unconjugated
• Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C to -70°C for longer.
  Dilute only prior to immediate use.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Database Link:
  Entrez Gene 54739 Human
  UniProt ID Q6GPH4
• Background: XAF-1 binds to XIAP, an inhibitor of caspases-3, -7, and -9, and triggers its relocation from the cytosol to the nucleus. Overexpression of XAF-1 results in the neutralization of XIAP’s ability to inhibit cell death. XAF-1 is normally expressed in all adult and fetal tissues but was found to be present in very low levels in a variety of cancer cell lines. In contrast, XIAP levels have been shown to be high in a majority of cell lines. Low XAF-1 and high basal expression of XIAP may therefore play a critical role in maintaining survival of cancer cell lines. Both IFN-a2 and IFN-b can induce XAF-1 mRNA in all cells examined but induction of XAF-1 protein (as observed by immunoblot analysis) was seen only in cell lines sensitive to the apoptotic effects of IFNs.
• Synonyms: XIAPAF1, BIRC4BP