Estrogen Receptor beta (ESR2) Rabbit Polyclonal Antibody

CAT#: AP06806PU-N

Estrogen Receptor beta (ESR2) rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot (WB) analysis of ER-beta antibody in extracts from HeLa cells.

Specifications

Product Data

• Applications: ELISA, IF, WB
• Recommended Dilution: Western blot: 1/500-1/1000.
  Immunofluorescence: 1/50-1/200.
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Synthetic peptide, corresponding to amino acids 71-120 of Human ERβ.
• Specificity: The antibody detects endogenous levels of ER-beta protein.
  (region surrounding Glu101)
• Formulation: Phosphate buffered saline (PBS), pH~7.2
  State: Aff - Purified
  State: Liquid purified Ig fraction (> 95% pure by SDS-PAGE).
  Preservative: 0.05% Sodium Azide
• Concentration: 1.0 mg/ml
• Purification: Affinity Chromatography using epitope-specific immunogen.
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: ~ 46, 60 kDa
• Gene Name: estrogen receptor 2
• Database Link:
  Entrez Gene 2100 Human
  UniProt ID Q92731
• Background: The discovery of a second estrogen receptor has redefined the estrogen signaling pathway and may have broad implications on estrogen-responsive tissues. The new estrogen receptor, named estrogen receptor-beta (ERβ), is preferentially expressed in the prostate and maintains some characteristics that are different from ERα.2 The rat tissue distribution and/or the relative level of ERα and ERβ expression seems to be quite different, i.e., moderate to high expression in uterus, testis, pituitary, ovary, kidney, epididymis, and adrenal for ERα and prostate, ovary, lung, bladder, brain, bone, uterus, and testis for ERβ. Within the same organ it often appears that the ER subtypes are expressed in different cell types, supporting the hypothesis that the ER's may have different biological functions. The discovery of ERβ suggests the existence of two previously unrecognized pathways of estrogen signaling, via the ERβ subtype in tissues exclusively expressing this subtype and via the formation of heterodimers in tissues expressing both ER subtypes.The existence of two ER subtypes, their differential expression pattern, and different actions on certain response elements could provide explanations for the striking species-, cell-, and promoter-specific actions of estrogens and antiestrogens.3 Both estrogen receptors appear to be involved in a multitude of regulatory events.
• Synonyms: ER-beta,ESR2, ESTRB, NR3A2