Cofilin 1 (CFL1) (+ Cofilin 2) Rabbit Polyclonal Antibody

CAT#: AP06770PU-N

Cofilin 1 (CFL1) (+ Cofilin 2) rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot (WB)analyzes of Cofilin1/2 antibody in extracts from Jurkat cells

Specifications

Product Data

• Applications: IF, WB
• Recommended Dilution: Western blot: 1/500-1/1000.
  Immunofluorescence: 1/50-1/200.
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Synthetic peptide, corresponding to amino acids 55-100 of Human Cofilin1.
• Specificity: This antibody detects endogenous levels of Cofilin1/2 protein.
  (region surrounding Thr88)
• Formulation: Phosphate buffered saline (PBS), pH 7.2.
  State: Aff - Purified
  State: Liquid purified Ig fraction
  Preservative: 0.05% sodium azide
• Concentration: 1.0 mg/ml
• Purification: Affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE)
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: ~ 19 kDa
• Gene Name: cofilin 1
• Database Link:
  Entrez Gene 12631 MouseEntrez Gene 29271 RatEntrez Gene 1072 Human
  UniProt ID P23528
• Background: Cofilin is ubiquitously expressed in eukaryotic cells where it binds to Actin,thereby regulating the rapid cycling of Actin assembly and disassembly essential for cellular viability. Cofilin is a low molecular weight protein that binds to filamentous F-Actin by bridging two longitudinally associated Actin subunits changing the F-Actin filament twist. This process is allowed by the dephosphorylation of Cofilin Ser 3 by factors such as opsonized zymosan.Lim kinase 1, a serine kinase, phosphorylates Cofilin and renders it unable to bind and depolymerise F-Actin.
• Synonyms: CFL1, CFL, p18

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Axon guidance, Fc gamma R-mediated phagocytosis, Regulation of actin cytoskeleton